| 产品编号 | bsm-33039M |
| 英文名称 | Mouse Anti-alpha Tubulin antibody |
| 中文名称 | 微管蛋白α/Tubulin α/α-tubulin单克隆抗体 |
| 别 名 | Alpha tubulin 1; Alpha-tubulin 1; Detyrosinated alpha Tubulin; FLJ30169; H2 alpha; TBA4A_HUMAN; Testis specific alpha tubulin; Testis-specific alpha-tubulin; TUBA 4A; TUBA1; Tuba4a; Tubulin alpha 1 (testis specific); Tubulin alpha 1; Tubulin alpha 1 chain; Tubulin alpha 4a; Tubulin alpha 4A chain; Tubulin alpha-1 chain; Tubulin alpha-4A chain; Tubulin H2 alpha; Tubulin H2-alpha; TUBA4A; α-tubulin; α tubulin; Tubulin-alpha. α tubulin; |
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Specific References (3) | bsm-33039M has been referenced in 3 publications.
[IF=6.513] Jinglei Wang. et al. Follicular fluid exosomes regulate OVGP1 secretion in yak oviduct epithelial cells via autophagy in vitro. J CELL PHYSIOL. 2023 Apr;: IF ; Bovine.
[IF=3.895] Shen X et al.Retinoic Acid-Induced Protein 14 (RAI14) Promotes mTOR-Mediated Inflammation Under Inflammatory Stress and Chemical Hypoxia in a U87 Glioblastoma Cell Line.(2018) Cell Mol Neurobiol. WB ; Human.
[IF=3.471] Yaying Wang. et al. Transcriptome sequencing reveals differences between leydig cells and sertoli cells of yak.. FRONT VET SCI. 2022 Aug;9:960250-960250 IF ; Bovine.
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| 研究领域 | 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 10F11 |
| 交叉反应 | Human,Mouse,Rat,Zebrafish (predicted: Rabbit,Pig,Chicken,Dog,GuineaPig) |
| 产品应用 | WB=1:50000-200000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 50kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | Recombinant human alpha Tubulin Protein |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulin. The genes encoding these microtubule constituents are part of the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes and they are highly conserved among and between species. This gene encodes an alpha tubulin that is a highly conserved homolog of a rat testis-specific alpha tubulin. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2013] Function: Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain Subunit: Dimer of alpha and beta chains. A typical microtubule is a hollow water-filled tube with an outer diameter of 25 nm and an inner diameter of 15 nM. Alpha-beta heterodimers associate head-to-tail to form protofilaments running lengthwise along the microtubule wall with the beta-tubulin subunit facing the microtubule plus end conferring a structural polarity. Microtubules usually have 13 protofilaments but different protofilament numbers can be found in some organisms and specialized cells. Subcellular Location: Cytoplasm, cytoskeleton Tissue Specificity: Widely expressed. Overexpressed in Platelet, Brain, and Frontal cortex Post-translational modifications: Some glutamate residues at the C-terminus are polyglutamylated. This modification occurs exclusively on glutamate residues and results in polyglutamate chains on the gamma-carboxyl group. Also monoglycylated but not polyglycylated due to the absence of functional TTLL10 in human. Monoglycylation is mainly limited to tubulin incorporated into axonemes (cilia and flagella) whereas glutamylation is prevalent in neuronal cells, centrioles, axonemes, and the mitotic spindle. Both modifications can coexist on the same protein on adjacent residues, and lowering glycylation levels increases polyglutamylation, and reciprocally. The precise function of such modifications is still unclear but they regulate the assembly and dynamics of axonemal microtubules (Probable). Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling (By similarity). Similarity: Belongs to the tubulin family. SWISS: P68366 Gene ID: 7277 Database links: Entrez Gene: 7277 Human Entrez Gene: 22145 Mouse Omim: 191110 Human SwissProt: P68366 Human SwissProt: P68368 Mouse Unigene: 75318 Human Unigene: 1155 Mouse Unigene: 92961 Rat
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| 产品图片 |
Sample:
Lane 1: Mouse Cerebrum tissue lysates
Lane 2: Mouse NIH/3T3 cell lysates
Lane 3: Rat Cerebrum tissue lysates
Lane 4: Human HeLa cell lysates
Lane 5: Human Jurkat cell lysates
Lane 6: Human A431 cell lysates
Lane 7: Human U251 cell lysates
Lane 8: Human 293T cell lysates
Lane 9: Human HepG2 cell lysates
Primary: Anti-alpha Tubulin (bsm-33039M) at 1/100000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution
Predicted band size: 50 kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (alpha Tubulin) Monoclonal Antibody, Unconjugated (ascites of bsm-33039M 10F11 ) at 1:2000 overnight at 4°C, followed by a conjugated secondary (sp-0024) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
