| 产品编号 | bsm-33151M |
| 英文名称 | Cleaved PARP1 |
| 中文名称 | 多腺苷二磷酸多聚酶/多聚ADP-核糖聚合酶1单克隆抗体 |
| 别 名 | ADP ribosyltransferase (NAD+; poly (ADP ribose) polymerase); ADP ribosyltransferase NAD+; ADPRT 1; ADPRT; ADPRT1; msPARP; NAD(+) ADP ribosyltransferase 1; pADPRT 1; pADPRT1; PARP 1; PARP; Poly (ADP ribose) polymerase 1; poly (ADP ribose) polymerase family, member 1; Poly adenosine diphosphate ADP ribose polymerase; Poly ADP ribose polymerase 1; Poly ADP ribose polymerase family member 1; Poly ADP ribose synthetase 1; poly(ADP ribose) synthetase; poly(ADP ribosyl)transferase; Poly[ADP ribose] synthetase 1; PPOL; sPARP 1; sPARP1; PARP1_HUMAN. |
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Specific References (1) | bsm-33151M has been referenced in 1 publications.
[IF=3.998] Hongbin Guo. et al. Metabolites profiling and pharmacokinetics of troxipide and its pharmacodynamics in rats with gastric ulcer. Sci Rep-Uk. 2020 Aug;10(1):1-21 IHC ; Rat.
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| 研究领域 | 肿瘤 心血管 细胞生物 信号转导 细胞凋亡 新陈代谢 线粒体 |
| 抗体来源 | Mouse |
| 克隆类型 | Monoclonal |
| 克 隆 号 | 4B1 |
| 交叉反应 | Human |
| 产品应用 | IHC-P=1:200-800, IHC-F=1:500-1000, IF=1:500-1000, ELISA=1:200-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 89/116kDa |
| 细胞定位 | 细胞核 细胞浆 细胞膜 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PARP |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]. Function: Involved in the base excision repair (BER) pathway, by catalyzing the poly(ADP-ribosyl)ation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism. This modification follows DNA damages and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks. Mediates the poly(ADP-ribosyl)ation of APLF and CHFR. Positively regulates the transcription of MTUS1 and negatively regulates the transcription of MTUS2/TIP150. With EEF1A1 and TXK, forms a complex that acts as a T-helper 1 (Th1) cell-specific transcription factor and binds the promoter of IFN-gamma to directly regulate its transcription, and is thus involved importantly in Th1 cytokine production. Subunit: Component of a base excision repair (BER) complex, containing at least XRCC1, PARP2, POLB and LRIG3. Homo- and heterodimer with PARP2. Interacts with PARP3, APTX and SRY. The SWAP complex consists of NPM1, NCL, PARP1 and SWAP70. Interacts with TIAM2 and ZNF423 (By similarity). Interacts (when poly-ADP-ribosylated) with CHD1L. Interacts with the DNA polymerase alpha catalytic subunit POLA1; this interaction functions as part of the control of replication fork progression. Interacts with EEF1A1, RNF4 and TXK. Subcellular Location: Mitochondrion outer membrane; Single-pass membrane protein. Nucleus membrane; Single-pass membrane protein. Endoplasmic reticulum membrane; Single-pass membrane protein. Nucleus. Post-translational modifications: Phosphorylated by PRKDC and TXK. Phosphorylated upon DNA damage, probably by ATM or ATR. Poly-ADP-ribosylated by PARP2. Poly-ADP-ribosylation mediates the recruitment of CHD1L to DNA damage sites. S-nitrosylated, leading to inhibit transcription regulation activity. Similarity: Contains 1 BRCT domain. Contains 1 PARP alpha-helical domain. Contains 1 PARP catalytic domain. Contains 2 PARP-type zinc fingers. SWISS: P09874 Gene ID: 142 Database links: Entrez Gene: 142 Human Entrez Gene: 11545 Mouse Omim: 173870 Human SwissProt: P09874 Human SwissProt: P11103 Mouse Unigene: 177766 Human Unigene: 277779 Mouse Unigene: 11327 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Cleaved PARP) Monoclonal Antibody, Unconjugated (bsm-33151M 4B1) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
