| 产品编号 | bs-20747R |
| 英文名称 | GPR49/LGR5 |
| 中文名称 | G蛋白偶联受体49抗体 |
| 别 名 | G protein coupled receptor 49; G-protein coupled receptor 49; G-protein coupled receptor 67; GPCR GPR49; G protein coupled receptor 67; FEX; g protein-coupled receptor fex; G-protein coupled receptor HG38; GPR 49; GPR 67; GPR67; Leucine rich repeat containing G protein coupled receptor 5; HG 38; HG38; LGR5; LGR5_HUMAN; MGC117008; Leucine-rich repeat-containing G-protein coupled receptor 5; LGR 5; Orphan G protein coupled receptor HG38. |
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Specific References (3) | bs-20747R has been referenced in 3 publications.
[IF=6.208] Ling Ding. et al. Bufalin Inhibits Tumorigenesis, Stemness, and Epithelial–Mesenchymal Transition in Colorectal Cancer through a C-Kit/Slug Signaling Axis. INT J MOL SCI. 2022 Jan;23(21):13354 WB ; Human.
[IF=5.076] Liu Yahong. et al. Preclinical Evaluation of Safety, Pharmacokinetics, Efficacy, and Mechanism of Radioprotective Agent HL-003. Oxid Med Cell Longev. 2021;2021:6683836 IHC ; Mouse.
[IF=4.175] Chaojie Li. et al. Hypoxia-induced Tie1 drives stemness and cisplatin resistance in non-small cell lung carcinoma cells. Cancer Cell Int. 2021 Dec;21(1):1-12 WB ; Human.
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| 研究领域 | 肿瘤 信号转导 干细胞 细胞凋亡 G蛋白偶联受体 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, Flow-Cyt=1μg/Test, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 98kDa |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human GPR49/LGR5: 211-310/907 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015] Function: Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. In contrast to classical G-protein coupled receptors, does not activate heterotrimeric G-proteins to transduce the signal. Involved in the development and/or maintenance of the adult intestinal stem cells during postembryonic development. Subunit: Identified in a complex composed of RNF43, LGR5 and RSPO1. Subcellular Location: Cell membrane; Multi-pass membrane protein Tissue Specificity: Expressed in skeletal muscle, placenta, spinal cord, and various region of brain. Expressed at the base of crypts in colonic and small mucosa stem cells. In premalignant cancer expression is not restricted to the cript base. Overexpressed in cancers of the ovary, colon and liver. Similarity: Belongs to the G-protein coupled receptor 1 family. Contains 16 LRR (leucine-rich) repeats. Contains 1 LRRNT domain. SWISS: O75473 Gene ID: 8549 Database links: Entrez Gene: 8549 Human Entrez Gene: 14160 Mouse Omim: 606667 Human SwissProt: O75473 Human SwissProt: Q9Z1P4 Mouse Unigene: 658889 Human Unigene: 42103 Mouse Unigene: 214063 Rat Lgr5基因（Wnt细胞信号系统）是一种G蛋白偶联受体，已经被确定为几种成年组织和癌症中干细胞的一个独特标记。Lgr5最初是在结肠癌细胞中发现的，有报道称：在恶化前的小鼠腺瘤中也有发现，这说明Lgr5很可能也是其他组织成体干细胞和癌症干细胞的标志物。 |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Stomach (Mouse) Lysate at 40 ug Lane 3: Colon (Mouse) Lysate at 40 ug Primary: Anti-GPR49/LGR5 (bs-20747R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 100 kD Observed band size: 105 kD 
Sample:
Brain (Mouse) Lysate at 40 ug Brain (Rat) Lysate at 40 ug Primary: Anti-LGR5 (bs-20747R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 98 kD Observed band size: 100 kD 
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (bs-20747R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse placenta tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (GPR49) Polyclonal Antibody, Unconjugated (bs-20747R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control (blue line): U251(fixed with pre-warmed 4% paraformaldehyde for 30min at 37℃)
Primary Antibody (green line): Rabbit Anti-GPR49 antibody (bs-20747R),Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC,Dilution: 1μg /test. |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
