| 产品编号 | bs-20697R |
| 英文名称 | Rabbit Anti-IL-1R1 antibody |
| 中文名称 | 白介素1受体1抗体 |
| 别 名 | Antigen CD121a; CD121 antigen-like family member A; CD121A; CD121a antigen; D2S1473; IL 1; IL 1R 1; IL 1R alpha; IL 1RT1; IL-1R-1; IL-1R-alpha; IL-1RT-1; IL-1RT1; IL1 inhibitor; IL1R; IL1R1; IL1R1_HUMAN; IL1RA; IL1RN; IL1RT1; Interleukin 1 receptor alpha type 1; Interleukin 1 receptor antagonist protein (IRAP); Interleukin 1 receptor type I; Interleukin receptor 1; Interleukin-1 receptor alpha; Interleukin-1 receptor type 1; Interleukin-1 receptor type I; P80; IL1 Receptor I; IL-1 Receptor I; Interleukin-1 receptor type 1, soluble form; IL-1 sRI. |
| 研究领域 | 心血管 免疫学 干细胞 t-淋巴细胞 细胞因子 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Pig,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1μg /test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 63kDa |
| 细胞定位 | 细胞膜 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human IL-1R1: 41-140/569 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cytokine receptor that belongs to the interleukin 1 receptor family. This protein is a receptor for interleukin alpha (IL1A), interleukin beta (IL1B), and interleukin 1 receptor, type I(IL1R1/IL1RA). It is an important mediator involved in many cytokine induced immune and inflammatory responses. This gene along with interleukin 1 receptor, type II(IL1R2), interleukin 1 receptor-like 2 (IL1RL2), and interleukin 1 receptor-like 1 (IL1RL1) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12. [provided by RefSeq]. Function: Receptor for IL1A, IL1B and IL1RN. After binding to interleukin-1 associates with the corecptor IL1RAP to form the high affinity interleukin-1 receptor complex which mediates interleukin-1-dependent activation of NF-kappa-B, MAPK and other pathways. Signaling involves the recruitment of adapter molecules such as TOLLIP, MYD88, and IRAK1 or IRAK2 via the respective TIR domains of the receptor/coreceptor subunits. Binds ligands with comparable affinity and binding of antagonist IL1RN prevents association with IL1RAP to form a signaling complex. Subunit: The interleukin-1 receptor complex is a heterodimer of IL1R1 and IL1RAP. Subcellular Location: Membrane; Single-pass type I membrane protein. Cell membrane (Probable). Secreted. Post-translational modifications: A soluble form (sIL1R1) is probably produced by proteolytic cleavage at the cell surface (shedding). Similarity: Belongs to the interleukin-1 receptor family. Contains 3 Ig-like C2-type (immunoglobulin-like) domains. Contains 1 TIR domain. SWISS: P14778 Gene ID: 3554 Database links: Entrez Gene: 3554 Human Entrez Gene: 16177 Mouse Omim: 147810 Human SwissProt: P14778 Human SwissProt: P13504 Mouse Unigene: 516249 Human Unigene: 701982 Human Unigene: 896 Mouse Unigene: 9758 Rat IL-1RⅠ属于新的信号受体家族Toll信号转导通路蛋白，IL-1RⅠ在免疫和炎症反应的研究中占重要地位，是在很多感染 、炎症性致病机制中起决定性作用的细胞因子，几乎可作用于全身各种细胞，IL-1 与IL-1R1的结合可导致大量与免疫反应和炎症相关基因的表达 ，包括细胞因子、细胞因子受体、急性期反应物、生长因子、胞外基质、粘附分子等。IL-1R1/NF-κB信号通路是机体免疫反应中十分重要的组成部分。白介素1受体I型 Interleukin 1 receptor I(IL-1RI) |
| 产品图片 |
Sample: Hela(human) Cell Lysate at 40 ug
Primary: Anti-IL-1R1 (bs-20697R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 63 kD
Observed band size: 63 kD
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (IL-1R1) Polyclonal Antibody, Unconjugated (bs-20697R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (IL-1R1) polyclonal Antibody, Unconjugated (bs-20697R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control (blue line): MCF7 (fixed with 70% methanol overnight at 4℃).
Primary Antibody (green line): Rabbit Anti-IL-1R1 receptor antibody (bs-20697R) ,Dilution: 0.2μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
