| 产品编号 | bs-21291R |
| 英文名称 | RBM19 |
| 中文名称 | RNA结合蛋白19抗体 |
| 别 名 | 1200009A02Rik; AV302714; NPO; Probable RNA-binding protein 19; RBM19; RBM19_HUMAN; RNA-binding motif protein 19. |
| 研究领域 | 信号转导 转录调节因子 结合蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 108kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RBM19: 1-100/960 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a nucleolar protein that contains six RNA-binding motifs. The encoded protein may be involved in regulating ribosome biogenesis. Multiple alternatively spliced variants, encoding the same protein, have been identified.[provided by RefSeq, Apr 2009] Function: Plays a role in embryo pre-implantation development. Subcellular Location: Nucleus > nucleolus. Nucleus > nucleoplasm. Cytoplasm. Chromosome. In discrete foci distributed throughout the cytoplasm and nucleoplasm during the 4 to 8 cell stages and the morula stage, but not in the periphery of the nucleolar precursor body (NPB). During blastocyst development, becomes increasingly localized to the nucleolus and less to the cytoplasm. At the late blastocyst stage, localized predominantly in the nucleolus. Localized in the nucleolus during interphase and to the perichromosomal sheath during mitosis. Does not colocalize in the cytoplasm with GW182 in P-bodies. May translocate to the nucleolus upon early embryonic development (By similarity). Colocalizes with NPM1 during interphase. By late prophase, metaphase, anaphase and telophase, associates with the chromosome periphery. By telophase localizes to NPB. Tissue Specificity: Expressed in the crypts of Lieberkuhn of the intestine and in intestinal neoplasia (at protein level). Similarity: Belongs to the RRM MRD1 family. Contains 6 RRM (RNA recognition motif) domains. SWISS: Q9Y4C8 Gene ID: 9904 Database links: Entrez Gene: 9904 Human Entrez Gene: 74111 Mouse SwissProt: Q9Y4C8 Human SwissProt: Q8R3C6 Mouse Unigene: 7482 Human |
| 产品图片 |
Sample:
A549(Human) Cell Lysate at 30 ug Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kD 
Sample:
K562(Human) Cell Lysate at 30 ug Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 108 kD Observed band size: 108 kD 
Sample:
HL60(Human) Cell Lysate at 30 ug Primary: Anti- RBM19 (bs-21291R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size:108 kD Observed band size: 110 kD |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
