| 产品编号 | bs-20494R |
| 英文名称 | CASP4 Rabbit pAb |
| 中文名称 | 半胱胺酸蛋白酶蛋白-4抗体 |
| 别 名 | Caspase-4 subunit 2; Caspase-4 subunit p10; Caspase-11; CASP-11; Protease ICH-3; Apoptosis related cysteine peptidase; Apoptotic cysteine protease Mih1/TX; CASP 4; CASP 4; CASP-4; CASP4; CASP4_HUMAN; CASP4_MOUSE; Caspase 4 apoptosis related cysteine peptidase; Caspase-4 subunit 2; Caspase4; ICE(rel) II; ICE(rel)-II; ICE(rel)II; ICEREL II; ICERELII; ICH 2; ICH 2 protease; ICH2; Mih1/TX; Mih1/TX; Protease ICH-2; Protease TX; TX; TX protease; EC:3.4.22.64. |
| 研究领域 | 肿瘤 细胞生物 干细胞 细胞凋亡 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Mouse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 10/43 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from mouse Caspase-4 subunit p10: 251-350/373 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Caspase 4 is a 377 amino acid protein (43 kDa) that belongs to the Ced3/ICE family of cystein proteases. Caspase 4 is an "upstream" caspase with a long prodomain. It undergoes a cleavage upon activation, leading to p20 and p10 subunits which have 67% homology to mature Caspase 1 over the equivalent coding sequences. Caspase 4 is capable of cleaving itself and the p30 Caspase 1 precursor, and of inducing apoptosis in transfected insect or COS cells. In high concentrations, it can also cleave the nuclear DNA enzyme PARP but the biological relevance of this cleavage is unclear. Caspase 4 mRNA is found in most tissues examined with the exception of the brain. Highest expression is found in spleen and lung, with moderate expression in heart and liver. Low expression is observed with skeletal muscle, kidney, and testis. Function: Involved in the activation cascade of caspases responsible for apoptosis execution. Cleaves caspase-1. Subunit: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a small and a large subunit. Tissue Specificity: Widely expressed, with highest levels in spleen and lung. Moderate expression in heart and liver, low expression in skeletal muscle, kidney and testis. Not found in the brain. Post-translational modifications: The two subunits are derived from the precursor sequence by an autocatalytic mechanism or by cleavage by Caspase-8. Similarity: Belongs to the peptidase C14A family. Contains 1 CARD domain. SWISS: P70343 Gene ID: 12363 Database links: Entrez Gene: 837 Human Entrez Gene: 12363 Mouse Omim: 602664 Human SwissProt: P49662 Human SwissProt: P70343 Mouse Unigene: 138378 Human Unigene: 1569 Mouse
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| 产品图片 |
Sample:
Lane 1: Rat Lymph node tissue lysates
Lane 2: Rat Lung tissue lysates
Primary: Anti-CASP4 (bs-20494R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 10/43 kDa
Observed band size: 47 kDa
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
