产品编号 | bs-20533R |
英文名称 | Rabbit Anti-PLA2G2A antibody |
中文名称 | 分泌型磷脂酶A2抗体 |
别 名 | PA2GA_HUMAN; Phospholipase A2, membrane associated; EC:3.1.1.4; GIIC sPLA2; Group IIA phospholipase A2; Non-pancreatic secretory phospholipase A2 (NPS-PLA2); Phosphatidylcholine 2-acylhydrolase 2A; PLA2B; PLA2L; RASF-A; phospholipase A2 group IIA; MOM1; PLA2; PLA2S; PLAS1; sPLA2; |
研究领域 | 细胞生物 细胞膜蛋白 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human) |
产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 14kDa |
细胞定位 | 细胞膜 分泌型蛋白 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PLA2G2A: 51-100/144 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4 |
保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The protein encoded by this gene is a member of the phospholipase A2 family (PLA2). PLA2s constitute a diverse family of enzymes with respect to sequence, function, localization, and divalent cation requirements. This gene product belongs to group II, which contains secreted form of PLA2, an extracellular enzyme that has a low molecular mass and requires calcium ions for catalysis. It catalyzes the hydrolysis of the sn-2 fatty acid acyl ester bond of phosphoglycerides, releasing free fatty acids and lysophospholipids, and thought to participate in the regulation of the phospholipid metabolism in biomembranes. Several alternatively spliced transcript variants with different 5' UTRs have been found for this gene.[provided by RefSeq, Sep 2009] Function: Thought to participate in the regulation of the phospholipid metabolism in biomembranes including eicosanoid biosynthesis. Catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Subcellular Location: Membrane. Similarity: Belongs to the phospholipase A2 family. SWISS: P14555 Gene ID: 5320 Database links: Entrez Gene: 5320 Human Entrez Gene: 18780 Mouse Omim: 172411 Human SwissProt: P14555 Human SwissProt: P31482 Mouse Unigene: 466804 Human Unigene: 4675 Mouse Unigene: 11346 Rat |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |