| 产品编号 | bs-20824R |
| 英文名称 | CK5+CK6 Rabbit pAb |
| 中文名称 | 细胞角蛋白5+6抗体 |
| 别 名 | CK5+6; Cytokeratin 5+6; Cytokeratin 5; Keratin, type II cytoskeletal 5; CK-5; Keratin-5; K5; 58 kDa cytokeratin; KRT5; CK 5; DDD; EBS 2; EBS2; K5; Keratin 5(epidermolysis bullosa simplex Dowling-Meara/Kobner/Weber-Cockayne types); Keratin 5; Keratin Type II Cytoskeletal 5; Keratin5; KRT 5; KRT 5A; KRT5.KRT6A; CK6A; CK6B; CK6C; CK6D; CK 6E; K6A; K6C; K6D; KRT6C; Cytokeratin 6a; Cytokeratin 6B; Cytokeratin 6C; Cytokeratin 6D; Cytokeratin 6E; Cytokeratin6; k6a keratin; K6B; K6b keratin; K6c keratin; K6d keratin; K6e keratin; k6hf; Keratin 6 isoform K6e; Keratin 6C; Keratin 6E; Keratin K6h; Keratin type II cytoskeletal 6A; Keratin type II cytoskeletal 6B; Keratin type II cytoskeletal 6C; Keratin type II cytoskeletal 6D; Keratin type II cytoskeletal 6E; KRT6; KRT6A; KRT6B; KRT6C; KRT6D; KRT6E; MGC102925. |
| 研究领域 | 肿瘤 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1μg/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 62/60 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human CK5+CK6: 161-220/590 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Keratins 5, 6 are members of type II keratins family that are specifically expressed in the inner root sheath of hair follicles. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin 18 is memeber of the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. SWISS: P13647 Gene ID: 3852 Database links: Entrez Gene: 3852 Human Entrez Gene: 3853 Human Entrez Gene: 3854 Human Omim: 148040 Human SwissProt: P02538 Human SwissProt: P04259 Human SwissProt: P13647 Human 细胞角蛋白5&6是高分子量细胞角蛋白，分子量分别为58KDa和56KDa。其表达在皮肤的基底细胞、棘层细胞和部分前列腺基底细胞。主要用于间皮瘤和腺癌的鉴别诊断，并可辅助诊断前列腺良恶性病变。 |
| 产品图片 |
Sample:
Esophagus (Mouse) Lysate at 40 ug
Primary: Anti-CK5+CK6 (bs-20824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/60 kD
Observed band size: 62/60 kD
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-CK5+CK6 (bs-20824R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 62/60 kD
Observed band size: 62/60 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (bs-20824R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (CK5+CK6) Polyclonal Antibody, Unconjugated (bs-20824R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control (blue line): Hela (fixed with 70% ethanol (Overmight at 4℃) and then permeabilized with 90% ice-cold methanol for 30 min at -20℃).
Primary Antibody (green line): Rabbit Anti-CK5+CK6 antibody (bs-20824R), Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody (white blue line): Goat anti-rabbit IgG-PE,Dilution: 1μg /test.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
