| 产品编号 | bs-20413R |
| 英文名称 | TGF-beta 3 Rabbit pAb |
| 中文名称 | 转移生长因子β3抗体（TGFβ3） |
| 别 名 | Transforming growth factor beta-3 proprotein; TGFB3; ARVD; FLJ16571; MGC105479; MGC118722; TGF beta 3; TGF beta3; TGF-beta-3; TGF-beta 3; TGFB 3; Transforming growth factor beta-3; TGFB3_HUMAN. |
| 研究领域 | 信号转导 干细胞 生长因子和激素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat) |
| 产品应用 | WB=1:500-2000,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30/47 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TGF-beta 3: 321-412/412 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The TGFBeta superfamily is composed of numerous growth and differentiation factors, including transforming growth factors Beta(TGFBeta) 1,2 and 3; placental transforming growth factor (PTGFBeta);growth /differentiation factors (GDFs); mullerian inhibiting substance (MIS);bone morphogenic proteins (BMPs);glial cell line-derived neurotrophic factor (GDNF);inhibins or activins (Alpha,Beta-A, Beta-B and Beta-C),Lefty and Nodal. Members of the TGF superfamily are involved in embryonic development and adult tissue homeostasis. Function: Involved in embryogenesis and cell differentiation. Subunit: Homodimer; disulfide-linked. Interacts with ASPN. Subcellular Location: Secreted. DISEASE: Defects in TGFB3 are a cause of familial arrhythmogenic right ventricular dysplasia type 1 (ARVD1) [MIM:107970]; also known as arrhythmogenic right ventricular cardiomyopathy 1 (ARVC1). ARVD is an autosomal dominant disease characterized by partial degeneration of the myocardium of the right ventricle, electrical instability, and sudden death. It is clinically defined by electrocardiographic and angiographic criteria; pathologic findings, replacement of ventricular myocardium with fatty and fibrous elements, preferentially involve the right ventricular free wall. Similarity: Belongs to the TGF-beta family. SWISS: P10600 Gene ID: 7043 Database links: Entrez Gene: 7043 Human Entrez Gene: 21809 Mouse Omim: 190230 Human SwissProt: P10600 Human SwissProt: P17125 Mouse Unigene: 592317 Human Unigene: 3992 Mouse Unigene: 7018 Rat 生长因子和激素（ Growth Factor and Hormones） TGF是一种多效生长因子,对上皮细胞增值有潜在抑制作用,可抑制肿瘤生长，用于许多恶性肿瘤如:胃癌、肺癌、膀胱癌、肾癌、前列腺癌、结肠癌等多种恶性肿瘤的研究。分子量：47kDa。此抗体是TGFβ3 特异性抗体，与TGF-β1和TGF-β2没有交叉反应。 |
| 产品图片 |
Sample:Ovary (Mouse)Lysate at 40 ug
Primary: Anti-TGF-beta 3(bs-20413R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 30'47kD
Observed band size: 47kD
Blank control（black line）:SH-SY5Y.
Primary Antibody (green line): Rabbit Anti-TGF-beta 3 antibody (bs-20413R)
Dilution:1ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-AF488
Dilution: 0.5ug/Test.
Negative control（white blue line）: PBS
Isotype control（orange line）: Normal Rabbit IgG
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at -20℃.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
