| 产品编号 | bs-16457R |
| 英文名称 | phospho-PFKM/PFK1 (Ser775) Rabbit pAb |
| 中文名称 | 磷酸化肌肉型6磷酸果糖激酶/磷酸果糖激酶1抗体 |
| 别 名 | PFKM(phospho S775); PFKM(phospho Ser775); p-PFKM(Ser775); Fructose 6 Phosphate Kinase; 6 phosphofructokinase Muscle Type; GSD7; PFKA; PFK-A; PFKP; PFKX; phosphofructo 1 Kinase Isozyme A; phosphofructo-1-kinase isozyme A; phosphofructokinase 1; phosphofructokinase M; phosphofructokinase-M; phosphofructokinase, muscle; phosphofructokinase, muscle type; phosphofructokinase, polypeptide X; phosphohexokinase; PFKAM_HUMAN; 6-phosphofructokinase, muscle type; PFK1; PFK-1; ATP-PFK; PPP1R122. |
| 产品类型 | 磷酸化抗体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Pig,Sheep,Cow,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 86 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated Synthesised phosphopeptide derived from human PFKM around the phosphorylation site of Ser775: KR(p-S)GE |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Three phosphofructokinase isozymes exist in humans: muscle, liver and platelet. These isozymes function as subunits of the mammalian tetramer phosphofructokinase, which catalyzes the phosphorylation of fructose-6-phosphate to fructose-1,6-bisphosphate. Tetramer composition varies depending on tissue type. This gene encodes the muscle-type isozyme. Mutations in this gene have been associated with glycogen storage disease type VII, also known as Tarui disease. Alternatively spliced transcript variants have been described.[provided by RefSeq, Nov 2009] Function: Catalyzes the third step of glycolysis, the phosphorylation of fructose-6-phosphate (F6P) by ATP to generate fructose-1,6-bisphosphate (FBP) and ADP. Subunit: Homo- and heterotetramers. Muscle is M4, liver is L4, and red cell is M3L, M2L2, or ML3. Interacts (via C-terminus) with HK1 (via N-terminal spermatogenic cell-specific region) Subcellular Location: Cytoplasm. Post-translational modifications: GlcNAcylation decreases enzyme activity. DISEASE: Glycogen storage disease 7 (GSD7) [MIM:232800]: A metabolic disorder characterized by exercise intolerance with associated nausea and vomiting, muscle cramping, exertional myopathy and compensated hemolysis. Short bursts of intense activity are particularly difficult. Severe muscle cramps and myoglobinuria develop after vigorous exercise. Similarity: Belongs to the phosphofructokinase type A (PFKA) family. ATP-dependent PFK group I subfamily. Eukaryotic two domain clade 'E' sub-subfamily. SWISS: P08237 Gene ID: 5213 Database links: Entrez Gene: 100034116 Horse Entrez Gene: 5213 Human Entrez Gene: 18642 Mouse Entrez Gene: 100345647 Rabbit Omim: 610681 Human SwissProt: Q867C9 Horse SwissProt: P08237 Human SwissProt: P47857 Mouse SwissProt: P00511 Rabbit Unigene: 75160 Human Unigene: 272582 Mouse Unigene: 11004 Rat |
| 产品图片 |
Sample:
Lane 1: Human A431 cell lysates
Lane 2: Human HeLa cell lysates
Lane 3: Human Raji cell lysates
Lane 4: Human SH-SY5Y cell lysates
Lane 5: Human LOVO cell lysates
Lane 6: Human MCF-7 cell lysates
Primary: Anti-phospho-PFKM/PFK1 (Ser775) (bs-16457R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 86 kDa
Observed band size: 77 kDa
Tissue/cell: Rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-phospho-PFKL (Ser775) Polyclonal Antibody, Unconjugated(bs-16457) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
