| 产品编号 | bs-21221R |
| 英文名称 | SLC25A18 Rabbit pAb |
| 中文名称 | 溶质载体家族25成员18抗体 |
| 别 名 | GC2; 1500015I14Rik; GHC2_HUMAN; SLC25A18; GC-2; Glutamate/H(+) symporter 2; Solute carrier family 25 member 18; GHC2_MOUSE; GHC2_RAT; |
| 研究领域 | 肿瘤 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse,Rat |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 34 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SLC25A18: 1-100/315 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
SLC25A18 belongs to the mitochondrial carrier family and contains three Solcar repeats. It is involved in the transport of glutamate across the inner mitochondrial membrane. Glutamate is co-transported with H(+). Function: Involved in the transport of glutamate across the inner mitochondrial membrane. Glutamate is cotransported with H+. Subcellular Location: Mitochondrion inner membrane; Multi-pass membrane protein. Similarity: Belongs to the mitochondrial carrier (TC 2.A.29) family. [View classification]. Contains 3 Solcar repeats. SWISS: Q9H1K4 Gene ID: 83733 Database links: Entrez Gene: 83733 Human Entrez Gene: 71803 Mouse Omim: 609303 Human SwissProt: Q9H1K4 Human SwissProt: Q9DB41 Mouse |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-SLC25A18 (bs-21221R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 34 kD
Observed band size: 34 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SLC25A18) Polyclonal Antibody, Unconjugated (bs-21221R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Blank control: THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A18 antibody (bs-21221R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
