| 产品编号 | bs-21160R |
| 英文名称 | RASAL2 Rabbit pAb |
| 中文名称 | RASAL2蛋白抗体 |
| 别 名 | nGAP; NGAP_HUMAN; Ras GTPase-activating protein nGAP; Ras protein activator like 1; RAS protein activator-like 2; RASAL2. |
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Specific References (1) | bs-21160R has been referenced in 1 publications.
[IF=5.646] Zhang W et al. IPO5 promotes the proliferation and tumourigenicity of colorectal cancer cells by mediating RASAL2 nuclear transportation. J Exp Clin Cancer Res. 2019 Jul 9;38(1):296. IHC ; Human.
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| 研究领域 | 细胞生物 信号转导 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse |
| 产品应用 | WB=1:500-2000,Flow-Cyt=2ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 129 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human RASAL2: 611-710/1139 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a protein that contains the GAP-related domain (GRD), a characteristic domain of GTPase-activating proteins (GAPs). GAPs function as activators of Ras superfamily of small GTPases. The protein encoded by this gene is able to complement the defective RasGAP function in a yeast system. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008] Function: Inhibitory regulator of the Ras-cyclic AMP pathway. Similarity: Contains 1 C2 domain. Contains 1 PH domain. Contains 1 Ras-GAP domain. SWISS: Q9UJF2 Gene ID: 9462 Database links: Entrez Gene: 9462 Human Omim: 606136 Human SwissProt: Q9UJF2 Human Unigene: 496139 Human |
| 产品图片 |
Sample:
Lane 1: Hela (Human) Cell Lysate at 30 ug
Lane 2: MDA-MB-231 (Human) Cell Lysate at 30 ug
Lane 3: MKN45 (Human) Cell Lysate at 30 ug
Lane 4: HepG2 (Human) Cell Lysate at 30 ug
Primary: Anti-RASAL2 (bs-21160R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 129 kD
Observed band size: 170 kD
Blank control: A431.
Primary Antibody (green line): Rabbit Anti-RASAL2 antibody (bs-21160R)
Dilution: 2ug/Test;
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 0.5ug/Test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
