| 产品编号 | bs-4431R |
| 英文名称 | PTPN20 Rabbit pAb |
| 中文名称 | 蛋白酪氨酸磷酸酶非受体型20抗体 |
| 别 名 | bA42B19.1; hPTPN20; Protein tyrosine phosphatase non receptor type 20; PTN20_HUMAN; PTP Typ; PTP Typ; Ptpn20; PTPN20A; PTPN20B; Tyrosine-protein phosphatase non-receptor type 20. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Pig) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 48 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PTPN20: 261-360/420 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The product of this gene belongs to the family of classical tyrosine-specific protein tyrosine phosphatases. Many protein tyrosine phosphatases have been shown to regulate fundamental cellular processes. The encoded protein appears to be targeted to sites of actin polymerization. A pseudogene of this gene has been defined on chromosome 10. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2014] Function: Tyrosine-protein phosphatase targeted to sites of actin polymerization in response of varied extracellular stimuli. Has tyrosine phosphatase activity towards various tyrosyl phosphorylated substrates. Subcellular Location: Nucleus. Cytoplasm. Cytoplasm > cytoskeleton > centrosome. Colocalizes with the microtubule-organizing center and intracellular membrane compartments. Tissue Specificity: Present in many cell lines (at protein level). Widely expressed. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the protein-tyrosine phosphatase family. Non-receptor class subfamily. Contains 1 tyrosine-protein phosphatase domain. SWISS: Q4JDL3 Gene ID: 653129 Database links: Entrez Gene: 26095 Human Entrez Gene: 653129 Human Omim: 610630 Human Omim: 610631 Human SwissProt: Q4JDL3 Human Unigene: 440733 Human Unigene: 499552 Human |
| 产品图片 |
Sample:
Testis (Mouse) Lysate at 40 ug
Primary: Anti-PTPN20 (bs-4431R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 48 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PTPN20) Polyclonal Antibody, Unconjugated (bs-4431R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
