产品编号 | bs-0432R-HRP |
英文名称 | Rabbit Anti-Chicken IgY antibody |
中文名称 | 辣根过氧化物酶标记兔抗鸡IgY抗体 |
别 名 | Rabbit Anti-Chicken IgY H&L (HRP) |
Specific References (2) | bs-0432R-HRP has been referenced in 2 publications.
[IF=4.02] Xu et al. A Critical Role of Bacterioferritin in Salmonella pullorum-Induced IFN-β Expression in DF-1 Cells. (2016) Front.Microbiol. 7:20 WB ; Salmonella.
[IF=1.73] Liu, Dong, and Zhong-Xiang Niu. "Cloning of a gene fragment encoding chicken complement component C3d with expression and immunogenicity of Newcastle disease virus F gene?CC3d fusion protein."Avian Pathology 37.5 (2008): 477-485.i WB ; Chicken.
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抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Chicken |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ELISA=1:500-1000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
性 状 | Liquid |
浓 度 | 1.0 mg/ml |
免 疫 原 | Native Chicken IgY |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.03% Proclin300 and 50% glycerol. |
保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
产品介绍 |
In chickens, immunoglobulin Y is the functional equivalent to Immunoglobulin G (IgG). Like IgG, it is composed of two light and two heavy chains. Structurally, these two types of immunoglobulin differ primarily in the heavy chains, which in IgY have a molecular mass of about 65,100 atomic mass units (amu), and are thus larger than in IgG. The light chains in IgY, with a molar mass of about 18,700 amu, are somewhat smaller than the light chains in IgG. The molar mass of IgY thus amounts to about 167,000 amu. The steric flexibility of the IgY molecule is less than that of IgG.Functionally, IgY is partially comparable to Immunoglobulin E (IgE), as well as to IgG. However, in contrast to IgG, IgY does not bind to Protein A, to Protein G, or to cellular Fc receptors. Furthermore, IgY does not activate the complement system. The name Immunoglobulin Y was suggested in 1969 by G.A. Leslie and L.W. Clem, after they were able to show differences between the immunoglobulins found in chicken eggs, and immunoglobulin G. Other synonymous names are Chicken IgG, Egg Yolk IgG, and 7S-IgG. |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |