| 产品编号 | bs-40296G-IRDye8 |
| 英文名称 | AffiniPure Goat Anti-Mouse IgG H&L(min X Hu, Bov, Hrs, Rb, Sw Sr Prot) antibody |
| 中文名称 | IRDye8标记的亲和纯化山羊抗小鼠IgG (H+L)（最小交叉反应：Hu, Bov, Hrs, Rb, Sw Sr Prot） |
| 别 名 | IRDye® 800CW Goat anti-Mouse IgG H+L; 山羊抗小鼠IgG H&L (IRDye® 800CW)预吸附二抗; |
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Specific References (6) | bs-40296G-IRDye8 has been referenced in 6 publications.
[IF=6.556] Bing Liu. et al. Core-shell SERS nanotags-based western blot. TALANTA. 2023 Feb;253:123888 Other ; nanoparticles.
[IF=3.491] Lili Han. et al. Long intergenic noncoding RNA smad7 (Linc‐smad7) promotes the epithelial‐mesenchymal transition of HCC by targeting the miR‑125b/SIRT6 axis. Cancer Med-Us. 2020 Dec;9(23):9123-9137 WB ; Human.
[IF=3.333] Han et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. (2018) Int.J.Oncol. 53:2433-2444 WB ; Human.
[IF=3.167] Zhenya Zhai. et al. Policosanol alleviates hepatic lipid accumulation by regulating bile acids metabolism in C57BL6/mice through AMPK–FXR–TGR5 cross-talk. 2021 Nov 03 IF ; Mouse.
[IF=1.817] Jiang Hai. et al. Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism. MEDICINE. 2022 Dec;101(52):e32541 WB ; Human.
[IF=1.772] Penggang Liu. et al. Expression of tumour transcription factor GLI1 in canine mammary tumours tissue. VET MED SCI. 2022 Jun 06 IF ; Dog.
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| 抗体来源 | Goat |
| 克隆类型 | Polyclonal |
| 产品应用 | WB=1:10000-25000,IF=1:200-1200
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1.0 mg/ml |
| 免 疫 原 | Native Mouse IgG |
| 亚 型 | IgG1 |
| 纯化方法 | affinity purified by Protein G |
| 缓 冲 液 | 10 mM TBS (pH=7.4) with 1% BSA, 0.01% Sodium Azide and 50% glycerol. |
| 保存条件 | Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
IRDye800CW Conjugated Goat (polyclonal) Anti-Mouse IgG(H+L), Highly Cross Adsorbed Producer: Li-Cor(USA) Product Number: 926-32210 Fluorophore: IRDye 800CW(MW: 1166) Excitation Wavelength: 778nm(in PBS) Emission Wacelegth: 795nm(in PBS) Fluorophore/Protein Ratio: 1.9 moles IRDye 800CW: 1mole IgG IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of mouse IgG as well as the light chains common to most mouse immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, bovine, horse, rabbit and swine serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications. |
| 产品图片 |
Sample:
Lane 1: Rat Cerebrum tissue lysates
Lane 2: Rat Heart tissue lysates
Lane 3: Human A549 cell lysates
Lane 4: Human Jurkat cell lysates
Lane 5: Human Huvec cell lysates
Lane 6: Human Hela cell lysates
Lane 7: Human U2os cell lysates
Lane 8: Human HepG2 cell lysates
Primary: Anti- GAPDH (bsm-33033M) at 1/50000 dilution
Secondary: IRDye800CW Goat Anti-Mouse IgG （bs-40296G-IRDye8）at 1/20000 dilution
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
