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IRDye® 800CW Goat Anti-Rabbit IgG H&L antibody (bs-40295G-IRDye8)

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说明书： 100ul

100ul/880.00元

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产品编号	bs-40295G-IRDye8
英文名称	IRDye® 800CW Goat Anti-Rabbit IgG H&L
中文名称	IRDye 800cw羊抗兔IgG (H+L)(进口分装)
别名	IRDye® 800CW Goat anti-Rabbit IgG H&L; Immunoglobulin G; 山羊抗兔IgG H&L (IRDye® 800CW)预吸附二抗; ®IRDye 800cw 羊抗兔IgG (H+L);
	Specific References (9) \| bs-40295G-IRDye8 has been referenced in 9 publications. [IF=6.706] Yunhua Zhang. et al. Hepatic G Protein-Coupled Receptor 180 Deficiency Ameliorates High Fat Diet-Induced Lipid Accumulation via the Gi-PKA-SREBP Pathway. NUTRIENTS. 2023 Jan;15(8):1838 WB ; Mouse. PubMed:37111058 [IF=5.075] Yuting Wu. et al. Construction, Characterization, and Application of a Nonpathogenic Virus-like Model for SARS-CoV-2 Nucleocapsid Protein by Phage Display. TOXINS. 2022 Oct;14(10):683 WB ; Other. PubMed:36287952 [IF=4.068] Ming Li. et al. ADAMTS12, a novel prognostic predictor, promotes cell proliferation, migration and invasion in head and neck squamous cell carcinoma. ORAL DIS. 2022 Oct;: WB ; Human. PubMed:36222542 [IF=3.707] Tushar A. Shah. et al. Therapeutic Hypothermia Inhibits the Classical Complement Pathway in a Rat Model of Neonatal Hypoxic-Ischemic Encephalopathy. Front Neurosci-Switz. 2021; 15: 616734 WB ; Rat. PubMed:33642979 [IF=3.333] Han et al. miR-103 promotes the metastasis and EMT of hepatocellular carcinoma by directly inhibiting LATS2. (2018) Int.J.Oncol. 53:2433-2444 WB ; Human. PubMed:30272278 [IF=3.167] Zhenya Zhai. et al. Policosanol alleviates hepatic lipid accumulation by regulating bile acids metabolism in C57BL6/mice through AMPK–FXR–TGR5 cross-talk. 2021 Nov 03 IF ; Mouse. PubMed:34730235 [IF=2.629] Zhu Wen-Bo. et al. Antipharyngitis Effects of Syringa oblata L. Ethanolic Extract in Acute Pharyngitis Rat Model and Anti-Inflammatory Effect of Ir-Idoids in LPS-Induced RAW 264.7 Cells. Evid-Based Compl Alt. 2021;2021:5111752 WB ; Rat. PubMed:34925529 [IF=1.817] Jiang Hai. et al. Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism. MEDICINE. 2022 Dec;101(52):e32541 WB ; Human. PubMed:36596012 [IF=1.772] Penggang Liu. et al. Expression of tumour transcription factor GLI1 in canine mammary tumours tissue. VET MED SCI. 2022 Jun 06 IF ; Dog. PubMed:35667035
抗体来源	Goat
克隆类型	Polyclonal
交叉反应	Rabbit
产品应用	WB=1:10000-30000 not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user.
性状	Liquid
浓度	1.0 mg/ml
免疫原	Native Rabbit IgG
亚型	IgG1
纯化方法	affinity purified by Protein G
缓冲液	10 mM TBS (pH=7.4) with 1% BSA, 0.01% Sodium Azide and 50% glycerol.
保存条件	Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项	This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍	IRDye800CW Conjugated Goat (polyclonal) Anti-Rabbit IgG(H+L), Highly Cross Adsorbed Producer: Li-Cor(USA) Product Number: 926-32211 Fluorophore: IRDye 800CW(MW: 1166) Excitation Wavelength: 778nm(in PBS) Emission Wacelegth: 795nm(in PBS) Fluorophore/Protein Ratio: 1.7 moles IRDye 800CW: 1mole IgG IRDye 800CW antibodies can be used for a variety of applications, including Western blotting, In-Cell Western assays, In-Gel Westerns, and many others. Isolation of specific antibodise was accomplished by immunoaffinity chromatography using antigens immobilized on agarose beads. Based on immunoelectrophoresis, this antibody reacts with the heavy chains of rabbit IgG as well as the light chains common to most rabbit immunoglobulins. No reactivity was detected against non-immunoglobulin serum proteins. This antibody was tested by ELISA and/or solid phase adsorbed for minimal cross-reactivity with human, mouse, and rat serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for Western blot and In-Cell Western applications.
产品图片	Sample: Lane 1,4,7: Mouse Testis tissue lysates Lane 2,5,8: Rat Testis tissue lysates Lane 3,6,9: Human MCF-7 cell lysates Primary: Anti-beta-Actin (bs-0061R) at 1/1:2000 dilution Secondary: IRDye800CW Goat anti-Rabbit IgG (bs-40295G-IRDye8) at 1:50000~200000 dilution Predicted band size: 42 kDa Observed band size: 42 kDa

	1、抗体溶解方法
	2、抗体修复方式
	3、常用试剂的配制
	4、免疫组化操作步骤
	5、免疫组化问题解答
	6、Western Blotting 操作步骤
	7、Western Blotting 问题解答
	8、关于肽链的设计
	9、多肽的溶解与保存
	10、酶标抗体效价测定程序