| 产品编号 | bs-20177R |
| 英文名称 | Rabbit Anti-ADRB1 antibody |
| 中文名称 | 肾上腺素能受体β1抗体 |
| 别 名 | beta 1 Adrenergic Receptor; beta 1 Adrenergic Receptor; ADRB1R; Adrenergic beta 1 receptor; B1AR; beta 1 adrenoceptor; beta 1 adrenoreceptor; beta-1 adrenergic receptor; beta-1 adrenoceptor; beta-1 adrenoreceptor; BETA1AR; RHR; ADR B1; ADRB 1; ADRB1; ADRB1_HUMAN; ADRB1R; Adrenergic beta 1 receptor; Adrenoceptor beta 1. |
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Specific References (2) | bs-20177R has been referenced in 2 publications.
[IF=5.955] Nguyen Khanh Toan. et al. Ascorbic acid induces salivary gland function through TET2/acetylcholine receptor signaling in aging SAMP1/Klotho (-/-) mice.. AGING-US. 2022 Aug;14(15):6028-6046 WB ; Mouse.
[IF=3.329] Enivwenaye E.W. Nabofa et al. Cardiovascular Effects of Caffeine in Rabbits Involve Beta-1 Adrenergic Receptor Activation. IHC ; rabbit.
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| 研究领域 | 心血管 神经生物学 信号转导 生长因子和激素 通道蛋白 细胞膜受体 细胞膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse,Rat (predicted: Pig,Dog) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,Flow-Cyt=1ug/Test
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ADRB1: 181-250/477 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | Preservative: 0.02% Proclin300, Constituents: 1% BSA, 0.01M PBS, pH7.4. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Beta-adrenergic receptors (Beta-1 adrenoreceptor) mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subcellular location cell membrane; Multi-pass membrane protein. Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Belongs to the G-protein coupled receptor 1 family. Function: Beta-adrenergic receptors mediate the catecholamine-induced activation of adenylate cyclase through the action of G proteins. This receptor binds epinephrine and norepinephrine with approximately equal affinity. Subunit: Interacts with GOPC, MAGI3 and DLG4. Subcellular Location: Cell membrane; Multi-pass membrane protein. Note=Localized at the plasma membrane. Found in the Golgi upon GOPC overexpression. Post-translational modifications: Homologous desensitization of the receptor is mediated by its phosphorylation by beta-adrenergic receptor kinase. Similarity: Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB1 sub-subfamily. SWISS: P08588 Gene ID: 153 Database links: Entrez Gene: 153 Human Entrez Gene: 11554 Mouse Omim: 109630 Human SwissProt: P08588 Human SwissProt: P34971 Mouse Unigene: 99913 Human Unigene: 46797 Mouse Unigene: 87064 Rat |
| 产品图片 |
Sample:
Lane 1: Stomach (Mouse) Lysate at 40 ug
Lane 2: Heart (Mouse) Lysate at 40 ug
Lane 3: Heart (Rat) Lysate at 40 ug
Primary: Anti-ADRB1 (bs-20177R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 50 kD
Paraformaldehyde-fixed, paraffin embedded (human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ADRB1) Polyclonal Antibody, Unconjugated (bs-20177R) at 1:400 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Blank control:U937.
Primary Antibody (green line): Rabbit Anti-ADRB1 antibody (bs-20177R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
