| 产品编号 | bs-4322R |
| 英文名称 | phospho-TBC1D4 (Ser588) Rabbit pAb |
| 中文名称 | TBC结构域家族D4蛋白抗体 |
| 别 名 | TBC1D4(phospho S588); p-TBC1D4(phospho S588); Acrg embryonic lethality(mouse) minimal region ortholog; Acrg embryonic lethality minimal region ortholog; Acrg embryonic lethality mouse minimal region ortholog; Akt substrate of 160 kDa; AS 160; AS160; BUB2; CDC16; DKFZp779C0666; KIAA0603; OTTHUMP00000040818; TBC(Tre 2 BUB2 CDC16) domain containing protein; TBC Tre 2 BUB2 CDC16 domain containing protein; TBC1 D4; TBC1 domain family member 4; Tbc1d4; TBCD4_HUMAN; Tre-2. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 信号转导 G蛋白信号 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Rabbit,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 60/147 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human TBC1D4 around the phosphorylation site of Ser588: LG(p-S)VD |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 | |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TBC1D4 (Ser588)) Polyclonal Antibody, Unconjugated (bs-4322R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
