产品编号 | bs-4490R |
英文名称 | Rabbit Anti-PLB1 antibody |
中文名称 | 磷脂酶B1膜相关蛋白抗体 |
别 名 | hPLB ; Lysophospholipase ; Phospholipase A2 ; Phospholipase B ; Phospholipase B/lipase ; Phospholipase B1 ; Phospholipase B1 membrane associated ; PLB 1 ; PLB ; PLB/LIP. |
研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | (predicted: Human,Mouse,Rat,Sheep,Cow) |
产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500,ICC/IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 163kDa |
细胞定位 | 细胞膜 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PLB1: 1001-1100/1458 <Extracellular> |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
PLB1 is a membrane-associated phospholipase which exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. It shows preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. It also exhibits esterase activity toward p-nitrophenyl. It may act on the brush border membrane to facilitate the absorption of digested lipids. Function: Membrane-associated phospholipase. Exhibits a calcium-independent broad substrate specificity including phospholipase A2/lysophospholipase activity. Preferential hydrolysis at the sn-2 position of diacylphospholipids and diacyglycerol, whereas it shows no positional specificity toward triacylglycerol. Exhibits also esterase activity toward p-nitrophenyl. May act on the brush border membrane to facilitate the absorption of digested lipids (By similarity). Subcellular Location: Apical cell membrane; Single-pass type I membrane protein. Note: Present in the intestinal brush border membranes. Tissue Specificity: Expressed in the epidermis (at protein level). Post-translational modifications: Undergoes proteolytic cleavage in the ileum. Similarity: Belongs to the 'GDSL' lipolytic enzyme family. Phospholipase B1 subfamily. SWISS: Q6P1J6 Gene ID: 151056 Database links: Entrez Gene: 151056 Human Omim: 610179 Human SwissProt: Q6P1J6 Human |
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |