| 产品编号 | bs-21073R |
| 英文名称 | p66 beta/GATAD2B |
| 中文名称 | p66β蛋白抗体 |
| 别 名 | C430014D17Rik; FLJ37346; GATA zinc finger domain containing 2B; GATA zinc finger domain containing protein 2B; GATA zinc finger domain-containing protein 2B; GATAD2B; KIAA1150; MGC138257; MGC138285; mKIAA1150; p66/p68; P66B_HUMAN; P66beta; RGD1308533; RP11-216N14.6; transcription repressor p66 beta component of the MeCP1 complex; Transcriptional repressor p66 beta; Transcriptional repressor p66-beta. |
| 研究领域 | 细胞生物 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human,Mouse (predicted: Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 66kDa |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human p66 beta: 381-480/593 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a zinc finger protein transcriptional repressor. The encoded protein is part of the methyl-CpG-binding protein-1 complex, which represses gene expression by deacetylating methylated nucleosomes. Mutations in this gene are linked to intellectual disability and dysmorphic features associated with mental retardation. [provided by RefSeq, Jun 2016] Function: Has transcriptional repressor activity. Targets MBD3 to discrete loci in the nucleus. Subcellular Location: Nucleus. Nuclear, in discrete foci. Tissue Specificity: Ubiquitous. Similarity: Contains 1 GATA-type zinc finger. SWISS: Q8WXI9 Gene ID: 57459 Database links: Entrez Gene: 57459 Human Entrez Gene: 229542 Mouse SwissProt: Q8WXI9 Human SwissProt: Q8VHR5 Mouse Unigene: 4779 Human Unigene: 596854 Human Unigene: 270999 Mouse Unigene: 133230 Rat |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug Hela(Human) Cell Lysate at 30 ug Primary: Anti-p66 beta (bs-21073R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 66 kD Observed band size: 66 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (p66 beta/GATAD2B) Polyclonal Antibody, Unconjugated (bs-21073R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
