产品编号 | bs-19988R |
英文名称 | PPME1 Rabbit pAb |
中文名称 | 蛋白磷酸酶PPME1抗体 |
别 名 | PME 1; PME-1; PME1; PPME1; PPME1_HUMAN; Protein phosphatase methylesterase 1. |
研究领域 | 肿瘤 细胞生物 信号转导 激酶和磷酸酶 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
克 隆 号 | |
交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Sheep,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 42 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human PPME1: 151-250/386 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
This gene encodes a protein phosphatase methylesterase localized to the nucleus. The encoded protein acts on the protein phosphatase-2A catalytic subunit and supports the ERK pathway through dephosphorylation of regulatory proteins. It plays a role in malignant glioma progression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2012] Function: Demethylates proteins that have been reversibly carboxymethylated. Demethylates PPP2CB (in vitro). Subunit: Binds PPP2CA and PPP2CB. Post-translational modifications: Phosphorylated by SIK1 following increases in intracellular sodium, leading to dissociation from the protein phosphatase 2A (PP2A) complex and subsequent dephosphorylation of sodium/potassium-transporting ATPase ATP1A1. Similarity: Belongs to the AB hydrolase superfamily. SWISS: Q9Y570 Gene ID: 51400 Database links: Entrez Gene: 51400 Human Entrez Gene: 72590 Mouse Omim: 611117 Human SwissProt: Q9Y570 Human SwissProt: Q8BVQ5 Mouse Unigene: 503251 Human Unigene: 177502 Mouse |
产品图片 |
Sample:Cerebrum (Mouse)Lysate at 40 ug
Primary: Anti-PPME1(bs-19988R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 42kD
Observed band size: 42kD
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1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |