| 产品编号 | bs-19963R |
| 英文名称 | PRKRIR Rabbit pAb |
| 中文名称 | 蛋白激酶PKR抑制蛋白PRKRIR抗体 |
| 别 名 | 52 kDa repressor of the inhibitor of the protein kinase; 58 kDa interferon induced protein kinase interacting protein; DAP4; Death associated protein 4; Inhibitor of protein kinase PKR; Interferon inducible double stranded RNA dependent inhibitor; MGC102750; P52rIPK; P58 repressor; p58IPK interacting protein; THAP domain containing protein 0; THAP0.P52K_HUMAN |
| 研究领域 | 细胞生物 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 88 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRKRIR: 301-400/761 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
PRKRIR is upstream regulator of interferon induced serine/threonine protein kinase R (PKR). It may block the PKR inhibitory function of P58IPK, resulting in the restoration of kinase activity and suppression of cell growth. Function: Upstream regulator of interferon-induced serine/threonine protein kinase R (PKR). May block the PKR-inhibitory function of P58IPK, resulting in restoration of kinase activity and suppression of cell growth. Subunit: Interacts with P58IPK, probably sequestring it. Subcellular Location: Nucleus Similarity: Contains 1 THAP-type zinc finger. SWISS: O43422 Gene ID: 5612 Database links: Entrez Gene: 5612 Human Entrez Gene: 72981 Mouse Omim: 607374 Human SwissProt: O43422 Human SwissProt: Q9CUX1 Mouse Unigene: 503315 Human |
| 产品图片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-PRKRIR (bs-19963R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 88 kD
Observed band size: 88 kD
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKRIR) Polyclonal Antibody, Unconjugated (bs-19963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRKRIR) Polyclonal Antibody, Unconjugated (bs-19963R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
