| 产品编号 | bs-19961R |
| 英文名称 | Prickle 2 Rabbit pAb |
| 中文名称 | 刺痛感蛋白2抗体 |
| 别 名 | PRIC2; PRIC2_HUMAN; prickle-like 2(Drosophila); prickle-like 2; Prickle-like protein 2; PRICKLE2. |
| 研究领域 | 细胞生物 染色质和核信号 干细胞 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 96 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Prickle 2: 401-500/844 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a homolog of Drosophila prickle. The exact function of this gene is not known, however, studies in mice suggest that it may be involved in seizure prevention. Mutations in this gene are associated with progressive myoclonic epilepsy type 5. [provided by RefSeq, Dec 2011] Subcellular Location: Nucleus membrane. Tissue Specificity: Expressed in brain, eye and testis. Additionally in fetal brain, adult cartilage, pancreatic islet, gastric cancer and uterus tumors. DISEASE: Epilepsy, progressive myoclonic 5. Similarity: Belongs to the prickle / espinas / testin family. Contains 3 LIM zinc-binding domains. Contains 1 PET domain. SWISS: Q7Z3G6 Gene ID: 166336 Database links: Entrez Gene: 166336 Human Entrez Gene: 243548 Mouse SwissProt: Q7Z3G6 Human SwissProt: Q80Y24 Mouse Unigene: 148105 Human Unigene: 699317 Human Unigene: 248820 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Prickle 2) Polyclonal Antibody, Unconjugated (bs-19961R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
