| 产品编号 | bs-19934R |
| 英文名称 | TINP1/NSA2 Rabbit pAb |
| 中文名称 | 转移生长因子诱导核蛋白1抗体 |
| 别 名 | HCLG1; TINP1; CDK105; HCL-G1; HUSSY29; HUSSY-29; Hairy cell leukemia protein 1; NSA2; NSA2_HUMAN; Ribosome biogenesis protein NSA2 homolog; TGF beta inducible nuclear protein 1; TGF-beta-inducible nuclear protein 1. |
| 研究领域 | 细胞生物 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TINP1/NSA2: 1-100/260 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a nucleolar protein involved in cell cycle regulation and proliferation. This gene was identified based on sequence similarity to a highly conserved Saccharomyces cerevisiae gene encoding a pre-ribosomal protein, which is involved in large ribosomal subunit biogenesis. The encoded protein is found at elevated levels in diabetic nephropathy. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified. [provided by RefSeq, Nov 2012] Function: Involved in the biogenesis of the 60S ribosomal subunit. May play a part in the quality control of pre-60S particles. Subunit: Component of the pre-66S ribosomal particle. Subcellular Location: Nucleus > nucleolus. Similarity: Belongs to the ribosomal protein S8e family. SWISS: O95478 Gene ID: 10412 Database links: Entrez Gene: 10412 Human Entrez Gene: 433230 Mouse Entrez Gene: 59050 Mouse Entrez Gene: 636306 Mouse SwissProt: O95478 Human SwissProt: Q9CR47 Mouse Unigene: 482526 Human Unigene: 390634 Mouse Unigene: 422907 Mouse Unigene: 129634 Rat Unigene: 15195 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TINP1/NSA2) Polyclonal Antibody, Unconjugated (bs-19934R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TINP1/NSA2) Polyclonal Antibody, Unconjugated (bs-19934R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
