| 产品编号 | bs-19928R |
| 英文名称 | phospho-SMC3 (Ser1083) Rabbit pAb |
| 中文名称 | 磷酸化基底膜相关软骨素蛋白多糖抗体 |
| 别 名 | SMC3(phospho S1083); p-SMC3(phospho S1083); CDLS2; DKFZp686L19178; DXhXs423e; DXS423E; KIAA0178; MGC138332; OTTHUMP00000061876; RP6 29D12.1; SB1.8; Segregation of mitotic chromosomes 1; Segregation of mitotic chromosomes like 1; SMC 1; SMC protein 1B; SMC-1-beta; SMC-1B; SMC1; SMC1A; SMC1alpha; SMC1alpha protein; SMC1B; SMC1B_HUMAN; SMC1BETA; SMC1beta protein; SMC1L1; SMC1L2; SMCB; Structural maintenance of chromosome 1 like 1 protein; Structural maintenance of chromosome 1 like 2 protein; Structural maintenance of chromosomes 1A; Structural maintenance of chromosomes 1B; Structural maintenance of chromosomes protein 1B. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 肿瘤 细胞生物 信号转导 细胞周期蛋白 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 141 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human SMC3 around the phosphorylation site of Ser1083: SG(p-S)QS- |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein. [provided by RefSeq, Jul 2008] Function: Central component of cohesin, a complex required for chromosome cohesion during the cell cycle. The cohesin complex may form a large proteinaceous ring within which sister chromatids can be trapped. At anaphase, the complex is cleaved and dissociates from chromatin, allowing sister chromatids to segregate. Cohesion is coupled to DNA replication and is involved in DNA repair. The cohesin complex plays also an important role in spindle pole assembly during mitosis and in chromosomes movement. Subcellular Location: Nucleus. Chromosome. Chromosome > centromere. Associates with chromatin. Before prophase it is scattered along chromosome arms. During prophase, most of cohesin complexes dissociate from chromatin probably because of phosphorylation by PLK, except at centromeres, where cohesin complexes remain. At anaphase, the RAD21 subunit of the cohesin complex is cleaved, leading to the dissociation of the complex from chromosomes, allowing chromosome separation. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Acetylation at Lys-105 and Lys-106 by ESCO1 is important for genome stability and S phase sister chromatid cohesion. Regulated by DSCC1, it is required for processive DNA synthesis, coupling sister chromatid cohesion establishment during S phase to DNA replication. DISEASE: Defects in SMC3 are the cause of Cornelia de Lange syndrome type 3 (CDLS3) [MIM:610759]. CDLS is a dominantly inherited multisystem developmental disorder characterized by growth and cognitive retardation, abnormalities of the upper limbs, gastroesophageal dysfunction, cardiac, ophthalmologic and genitourinary anomalies, hirsutism, and characteristic facial features. CDSL3 is a mild form with absence of major structural anomalies typically associated with CDLS. The phenotype in some instances approaches that of apparently non-syndromic mental retardation. Similarity: Belongs to the SMC family. SMC3 subfamily. SWISS: Q9UQE7 Gene ID: 9126 Database links: Entrez Gene: 395188 Chicken Entrez Gene: 9126 Human Entrez Gene: 13006 Mouse Entrez Gene: 399092 Xenopus laevis Entrez Gene: 324475 Zebrafish Omim: 606062 Human SwissProt: Q9UQE7 Human SwissProt: Q9CW03 Mouse SwissProt: O93309 Xenopus laevis Unigene: 24485 Human Unigene: 14910 Mouse Unigene: 11074 Rat Unigene: 290 Xenopus laevis Unigene: 75355 Xenopus laevis |
| 产品图片 |
Sample: LOVO Cell (Human) Lysate at 40 ug
Primary: Anti-p-SMC3(Ser1083) (bs-19928R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 141 kD
Observed band size: 135 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
