| 产品编号 | bs-19666R |
| 英文名称 | phospho-Thymidine Kinase 1 (Ser13) |
| 中文名称 | 磷酸化胸苷激酶1抗体 |
| 别 名 | Thymidine Kinase 1 (phospho S13); p-Thymidine Kinase 1 (phospho S13); cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2. |
| 产品类型 | 磷酸化抗体 |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Dog,Pig,Hamster) |
| 产品应用 | WB=1:500-2000, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 25kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthesised phosphopeptide derived from human Thymidine Kinase 1 around the phosphorylation site of Ser13: PG(pS)PS |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Thymidine Kinase (TK1) is a highly conserved phosphotransferase that is present in most living cells. Thymidine Kinase catalyzes the phosphorylation reaction: deoxythymidine + ATP = deoxythymidine 5'-phosphate + ADP; it is thus involved in the reaction chain to introduce deoxythymidine into the DNA. Thymidine kinase is required for the action of many antiviral drugs, such as azidothymidine (AZT), and is is also used to select hybridoma cell lines in the production of monoclonal antibodies. Thymidine Kinase has many clinical applications as it is only present in anticipation of cell division. Because of this, Thymidine Kinase can be used as a proliferation marker in the diagnosis, treatment, and follow-up of malignant diseases, especially hematological malignancies. Thymidine Kinase may be observed as a monomer, dimer, trimer or tetramer. Subcellular Location: Cytoplasm. Post-translational modifications: Phosphorylated on Ser-13 in mitosis. Similarity: Belongs to the thymidine kinase family. SWISS: P04183 Gene ID: 7083 Database links: Entrez Gene: 7083 Human Entrez Gene: 21877 Mouse Omim: 188300 Human SwissProt: P04183 Human SwissProt: P04184 Mouse Unigene: 515122 Human Unigene: 2661 Mouse Unigene: 195325 Rat Unigene: 216486 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
