| 产品编号 | bs-19639R |
| 英文名称 | OLFML2A Rabbit pAb |
| 中文名称 | 嗅介蛋白样蛋白2A抗体 |
| 别 名 | FLJ00237; Olfactomedin like 2A; Olfactomedin like protein 2A; OLM2A_HUMAN; Photomedin 1; PRO34319; UNQ9394/PRO34319. |
| 研究领域 | 细胞生物 神经生物学 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Zebrafish,Chicken,Dog,Cat,GuineaPig,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 65 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human OLFML2A: 501-600/652 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
OLFML2A contains one olfactomedin-like domain. Its exact function is unknown. There are three named isoforms produced by alternative splicing. Subunit: Homodimer. Binds to heparin and chondroitin sulfate E. Subcellular Location: Secreted Tissue Specificity: In the kidney expressed only by podocytes, wherein they localize to major processes. DISEASE: Detected at the vesicle stage of developing glomeruli, expressed in invading endothelial cells in the glomerular cleft at the S-shaped stage and is later expressed only at the basal aspect of maturing podocytes. SWISS: Q68BL7 Gene ID: 169611 Database links: Entrez Gene: 169611 Human Entrez Gene: 241327 Mouse SwissProt: Q68BL7 Human SwissProt: Q8BHP7 Mouse Unigene: 357004 Human Unigene: 103491 Mouse Unigene: 144357 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (OLFML2A) Polyclonal Antibody, Unconjugated (bs-19639R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
