产品编号 | bs-19619R |
英文名称 | SEC62 Rabbit pAb |
中文名称 | 转运蛋白SEC62抗体 |
别 名 | Dtrp1; FLJ32803; hTP-1; HTP1; Membrane protein SEC62, S.cerevisiae, homolog of; OTTHUMP00000213390; sec62; SEC62 homolog(S. cerevisiae); SEC62_HUMAN; TLOC1; TP-1; Translocation protein 1; Translocation protein sec62. |
研究领域 | 细胞生物 信号转导 转运蛋白 表观遗传学 |
抗体来源 | Rabbit |
克隆类型 | Polyclonal |
交叉反应 | Human,Mouse,Rat (predicted: Rabbit,Pig,Sheep,Cow,Dog,Horse) |
产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
理论分子量 | 46 kDa |
检测分子量 | |
细胞定位 | 细胞浆 |
性 状 | Liquid |
浓 度 | 1mg/ml |
免 疫 原 | KLH conjugated synthetic peptide derived from human SEC62: 251-350/399 |
亚 型 | IgG |
纯化方法 | affinity purified by Protein A |
缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
PubMed | PubMed |
产品介绍 |
The Sec61 complex is the central component of the protein translocation apparatus of the endoplasmic reticulum (ER) membrane. The protein encoded by this gene and SEC63 protein are found to be associated with ribosome-free SEC61 complex. It is speculated that Sec61-Sec62-Sec63 may perform post-translational protein translocation into the ER. The Sec61-Sec62-Sec63 complex might also perform the backward transport of ER proteins that are subject to the ubiquitin-proteasome-dependent degradation pathway. The encoded protein is an integral membrane protein located in the rough ER. [provided by RefSeq, Jul 2008] Function: Required for preprotein translocation. Subunit: Part of a complex that contains SEC61, SEC62 and SEC63. Interacts with SEC61B. Subcellular Location: Endoplasmic reticulum membrane. Similarity: Belongs to the SEC62 family. SWISS: Q99442 Gene ID: 7095 Database links: Entrez Gene: 7095 Human Entrez Gene: 69276 Mouse Omim: 602173 Human SwissProt: Q99442 Human SwissProt: Q8BU14 Mouse Unigene: 592561 Human |
产品图片 |
Sample:
Lane 1: Thymus (Mouse) Lysate at 40 ug
Lane 2: Uterus (Mouse) Lysate at 40 ug
Lane 3: Thymus (Rat) Lysate at 40 ug
Lane 4: A431 (Human) Cell Lysate at 30 ug
Primary: Anti- SEC62 (bs-19619R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 46 kD
Observed band size: 48 kD
|
1、抗体溶解方法 | |
2、抗体修复方式 | |
3、常用试剂的配制 | |
4、免疫组化操作步骤 | |
5、免疫组化问题解答 | |
6、Western Blotting 操作步骤 | |
7、Western Blotting 问题解答 | |
8、关于肽链的设计 | |
9、多肽的溶解与保存 | |
10、酶标抗体效价测定程序 | |