| 产品编号 | bs-19603R |
| 英文名称 | SDHAF1 Rabbit pAb |
| 中文名称 | 琥珀酸脱氢酶的组合因子1抗体 |
| 别 名 | LYR motif containing 8; LYR motif-containing protein 8; LYRM8; mitochondrial; SDH assembly factor 1; SDHAF1; SDHF1_HUMAN; Succinate dehydrogenase assembly factor 1; Succinate dehydrogenase assembly factor 1 mitochondrial; Succinate dehydrogenase complex assembly factor 1. |
| 研究领域 | 细胞生物 神经生物学 线粒体 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Pig,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 21 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SDHAF1: 1-100/115 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The succinate dehydrogenase (SDH) complex (or complex II) of the mitochondrial respiratory chain is composed of 4 individual subunits. The protein encoded by this gene resides in the mitochondria, and is essential for SDH assembly, but does not physically associate with the complex in vivo. Mutations in this gene are associated with SDH-defective infantile leukoencephalopathy (mitochondrial complex II deficiency).[provided by RefSeq, Mar 2010] Function: Plays an essential role in succinate dehydrogenase complex (SDH) assembly, a complex involved in complex II of the mitochondrial electron transport chain. Probably acts by participating in mitochondrial biosynthesis of iron-sulfur centers for complex II. Subcellular Location: Mitochondrion matrix. Tissue Specificity: Ubiquitously expressed. DISEASE: Mitochondrial complex II deficiency Similarity: Belongs to the complex I LYR family. SDHAF1 subfamily. SWISS: A6NFY7 Gene ID: 644096 Database links: Entrez Gene: 644096 Human Entrez Gene: 68332 Mouse Omim: 612848 Human SwissProt: A6NFY7 Human SwissProt: Q3U276 Mouse Unigene: 356460 Human Unigene: 6844 Mouse Unigene: 3527 Rat |
| 产品图片 |
Sample:
Jurkat(Human) Cell Lysate at 30 ug
Primary: Anti-SDHAF1 (bs-19603R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 21 kD
Observed band size: 22 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
