| 产品编号 | bs-7944R |
| 英文名称 | Rabbit Anti-Ubiquitin antibody |
| 中文名称 | 泛素蛋白抗体 |
| 别 名 | FLJ25987; MGC8385; RPS27A; RPS 27A; UBA52; UBA 52; UBA80; UBA 80; UBB; UBC; UB; UBCEP1; UBCEP2; UBCEP 1; UBCEP 2; UBCEP-1; UBCEP-2; Ubiquitin; ubiquitin B; UBC_HUMAN; UBB_HUMAN; RS27A_HUMAN; HMG20; Ubiquitin carboxyl extension protein 80; Ubiquitin; Polyubiquitin-C; Polyubiquitin-B. |
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Specific References (1) | bs-7944R has been referenced in 1 publications.
[IF=2.823] Peng‐fei Zhang. et al. Integrated analysis of phosphoproteome and ubiquitylome in epididymal sperm of buffalo (Bubalus bubalis). Mol Reprod Dev. 2021 Jan;88(1):15-33 WB,IP ; Bovine.
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| 研究领域 | 肿瘤 细胞生物 免疫学 神经生物学 细胞凋亡 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Sheep,Cow,Chicken) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,Flow-Cyt=1ug/Test,ICC/IF=1:100,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 8.5kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Ubiquitin: 63-74/76 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes ubiquitin, one of the most conserved proteins known. Ubiquitin has a major role in targeting cellular proteins for degradation by the 26S proteosome. It is also involved in the maintenance of chromatin structure, the regulation of gene expression, and the stress response. Ubiquitin is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin moiety fused to an unrelated protein. This gene consists of three direct repeats of the ubiquitin coding sequence with no spacer sequence. Consequently, the protein is expressed as a polyubiquitin precursor with a final amino acid after the last repeat. An aberrant form of this protein has been detected in patients with Alzheimer's disease and Down syndrome. Pseudogenes of this gene are located on chromosomes 1, 2, 13, and 17. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2013] Function: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Subcellular Location: Cytoplasm. Nucleus. Similarity: Belongs to the ubiquitin family. Contains 9 ubiquitin-like domains. SWISS: P0CG48 Gene ID: 7314 Database links: Entrez Gene: 100049794 Horse Entrez Gene: 7314 Human Entrez Gene: 22187 Mouse Omim: 191339 Human SwissProt: Q8MKD1 Horse SwissProt: P0CG47 Human SwissProt: P0CG48 Human SwissProt: P62988 Human SwissProt: P0CG49 Mouse SwissProt: P62991 Mouse Unigene: 356190 Human Unigene: 520348 Human Unigene: 282093 Mouse Unigene: 371592 Mouse Unigene: 110618 Rat Unigene: 1253 Rat Unigene: 3761 Rat 泛素及类泛素蛋白(Ubls) 存在在多种细胞中，有多种细胞调控的功能，如细胞增殖、凋亡、细胞周期及DNA修复等。但泛素途径的异常会导致人类疾病的产生，包括多种形式的肿瘤的发生，神经退变等都与泛素有关。 相关产品： Ubiquitin 1-76 peptides (bs-10803P); |
| 产品图片 |
Sample:
HepG2(Human) Cell Lysate at 30 ug
Primary: Anti-Ubiquitin (bs-7944R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 8.5 kD
Observed band size: 12 kD
Paraformaldehyde-fixed, paraffin embedded (human gastric carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Ubiquitin) Polyclonal Antibody, Unconjugated (bs-7944R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
HepG2 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (Ubiquitin) polyclonal Antibody, Unconjugated (bs-7944R) 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue, C02-04002) was used to stain the cell nuclei.
Blank control:Molt4.
Primary Antibody (green line): Rabbit Anti-Ubiquitin antibody (bs-7944R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-AF647
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at-20℃. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
