| 产品编号 | bs-19402R |
| 英文名称 | PRP18 homolog Rabbit pAb |
| 中文名称 | PRP18蛋白抗体 |
| 别 名 | FLJ10210; hPRP18; Pre mRNA splicing factor 18; Pre-mRNA-splicing factor 18; PRP18; PRP18 homolog; PRP18 pre mRNA processing factor 18 homolog(PRPF18); PRP18 pre mRNA processing factor 18 homolog(S. cerevisiae); PRP18_HUMAN; prpf18. |
| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Rabbit,Pig,Sheep,Cow,Zebrafish,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human PRP18 homolog: 281-342/342 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to the yeast splicing factor Prp18. [provided by RefSeq, Jul 2008] Function: Participates in the second step of pre-mRNA splicing. Subunit: Heterodimer with PPIH. Interacts with PRPF4 and with the spliceosome. Part of a complex containing U4/U6 snRNPs. Subcellular Location: Nucleus speckle. Colocalizes with spliceosomal snRNPs. Similarity: Belongs to the PRP18 family. SWISS: Q99633 Gene ID: 8559 Database links: Entrez Gene: 100727386 Guinea pig Entrez Gene: 100056558 Horse Entrez Gene: 8559 Human Entrez Gene: 67229 Mouse Entrez Gene: 100340516 Rabbit Omim: 604993 Human SwissProt: Q99633 Human SwissProt: Q8BM39 Mouse Unigene: 161181 Human Unigene: 38529 Mouse Unigene: 203914 Rat |
| 产品图片 |
Sample:
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-PRP18 homolog (bs-19402R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 40 kD
Paraformaldehyde-fixed, paraffin embedded (rat colon tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (PRP18 homolog) Polyclonal Antibody, Unconjugated (bs-19402R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
