| 产品编号 | bs-19384R |
| 英文名称 | SAP30 Rabbit pAb |
| 中文名称 | 组蛋白去乙酰化酶复合亚基SAP30抗体 |
| 别 名 | 30 kDa Sin3-associated polypeptide; Histone deacetylase complex subunit SAP 30; Histone deacetylase complex subunit SAP30; MSin3A associated polypeptide p30; SAP 30; Sap30; SAP30_HUMAN; Sin3 associated polypeptide 30 kDa; Sin3 associated polypeptide 30kD; Sin3 associated polypeptide; Sin3 corepressor complex subunit SAP 30; Sin3 corepressor complex subunit SAP30; Sin3-associated polypeptide p30; Sin3A associated protein 30kDa; Sin3A associated protein. |
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Specific References (1) | bs-19384R has been referenced in 1 publications.
[IF=3.385] Lvjing Luo. et al. SAP30 targeted by miR-133b was involved in the process of nuclear decondensation in Chinese mitten crab (Eriocheir sinensis) sperm. AQUACULT REP. 2023 Apr;29:101540 WB,IF ; Crab.
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| 研究领域 | 细胞生物 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Cow,Chicken,Dog,Cat,GuineaPig,Horse,Spider Monkey) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 23 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SAP30: 121-220/220 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Histone acetylation plays a key role in the regulation of eukaryotic gene expression. Histone acetylation and deacetylation are catalyzed by multisubunit complexes. The protein encoded by this gene is a component of the histone deacetylase complex, which includes SIN3, SAP18, HDAC1, HDAC2, RbAp46, RbAp48, and other polypeptides. This complex is active in deacetylating core histone octamers, but inactive in deacetylating nucleosomal histones. A pseudogene of this gene is located on chromosome 3. [provided by RefSeq, Jul 2008] Function: Involved in the functional recruitment of the Sin3-histone deacetylase complex (HDAC) to a specific subset of N-CoR corepressor complexes. Capable of transcription repression by N-CoR. Active in deacetylating core histone octamers (when in a complex) but inactive in deacetylating nucleosomal histones. Subcellular Location: Nucleus. Tissue Specificity: Expressed in all tissues tested with highest levels in pancreas, ovary, PBL, spleen and thymus; lowest levels in brain, placenta, lung and kidney. Similarity: Belongs to the SAP30 family. SWISS: O75446 Gene ID: 8819 Database links: Entrez Gene: 8819 Human Entrez Gene: 60406 Mouse Omim: 603378 Human SwissProt: O75446 Human SwissProt: O88574 Mouse Unigene: 591715 Human Unigene: 118 Mouse |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SAP30) Polyclonal Antibody, Unconjugated (bs-19384R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SAP30) Polyclonal Antibody, Unconjugated (bs-19384R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
