| 产品编号 | bs-19362R |
| 英文名称 | SACM1L |
| 中文名称 | 肌动蛋白抑制因子1抗体 |
| 别 名 | Phosphatidylinositide phosphatase SAC1; SAC1; SAC1 suppressor of actin mutations 1-like (yeast); SAC1_HUMAN; SACM1L; suppressor of actin 1; Suppressor of actin mutations 1-like protein. |
| 研究领域 | 细胞生物 信号转导 细胞骨架 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,Cat) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 67kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SACM1L: 431-530/587 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The Saccharomyces cerevisiae SAC1 gene modulates yeast actin function and alleviates the essential requirement for phosphatidylinositol transfer protein (sec14p) activity in Golgi secretory function. The SAC1 gene product (Sac1p) is an integral membrane lipid phosphatase of the endoplasmic reticulum (ER) and the Golgi complex and contains a Sac phosphatase domain (1-2). Sac1p functions in a wide range of cellular processes including inositol metabolism, actin cytoskeletal organization, endoplasmic reticulum ATP transport, phosphatidylinositol-phospha-tidylcholine transfer protein function and multiple-drug sensitivity (3). Sac1p is an important regulator of microsomal ATP transport, providing a link between inositol phospholipid signaling and ATP-dependent processes in the yeast ER (4). Defects in Sac1p relieves the requirement for Sec14p by altering phospholipid metabolism to expand the pool of diacylglycerol in the Golgi (5). Sac1p dysfunction exerts its pleiotropic effects on yeast Golgi function, the organization of the actin cytoskeleton, and the cellular requirement for inositol, through altered metabolism of inositol glycerophospholipids (6). These effects suggest the secretory and cytoskeletal activities are coordinated to achieve proper spatial regulation of secretion in S. cerevisiae (7). Function: Phosphoinositide phosphatase that hydrolyzes PtdIns(3)P and PtdIns(4)P. Has low activity towards PtdIns(3,5)P2. Subcellular Location: Endoplasmic reticulum membrane. Tissue Specificity: Detected in heart, brain, lung, liver, kidney, pancreas and testis. Similarity: Contains 1 SAC domain. SWISS: Q9NTJ5 Gene ID: 22908 Database links: Entrez Gene: 101787561 Guinea pig Entrez Gene: 100065601 Horse Entrez Gene: 22908 Human Entrez Gene: 83493 Mouse Entrez Gene: 791142 Zebrafish Omim: 606569 Human SwissProt: Q9NTJ5 Human SwissProt: Q9EP69 Mouse SwissProt: A1L244 Zebrafish Unigene: 156509 Human Unigene: 273671 Mouse Unigene: 161978 Rat |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
