| 产品编号 | bs-0365R |
| 英文名称 | human IgG Fc antibody |
| 中文名称 | 兔抗人IgG-Fc段抗体 |
| 别 名 | Immunoglobulin G Fc fragment; human IgG-Fc; human IgG Fc fragment. |
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Specific References (1) | bs-0365R has been referenced in 1 publications.
[IF=4.566] Su Yan. et al. The Circ-CYP24A1-miR-224-PRLR Axis Impairs Cell Proliferation and Apoptosis in Recurrent Miscarriage. Front Physiol. 2022 Mar;0:347 IHC ; Human.
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| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human |
| 产品应用 | WB=1:1000-3000,IHC-P=1:100-500,IHC-F=1:500-2000,IF=1:500-2000,ELISA=1:1000-6000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | human IgG-Fc purified from human plasma. |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M PBS (pH7.4). |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| 产品介绍 |
The Fc region is the constant region on an immunoglobulin molecule, the area that is exactly the same on all antibodies. The Fc region is found on the heavy chains and is not involved in binding antigens. SWISS: N/A Gene ID: N/A |
| 产品图片 |
Sample:
Lane 1: Human Serum
Primary: Anti-human IgG Fc (bs-0365R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: kDa
Observed band size: 50 kDa
Paraformaldehyde-fixed, paraffin embedded (human colon ); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (bs-0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human colon carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (bs-0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (human tonsil); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (human IgG Fc) Polyclonal Antibody, Unconjugated (bs-0365R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
