| 产品编号 | bs-19273R |
| 英文名称 | Rabbit Anti-BarX2 antibody |
| 中文名称 | BarX2蛋白抗体 |
| 别 名 | BarH like homeobox 2; BarX 2; BARX homeobox; BARX homeobox 2; Barx2; BARX2_HUMAN; Homeobox protein BarH like 2; Homeobox protein BarH-like 2; MGC133368; MGC133369. |
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Specific References (1) | bs-19273R has been referenced in 1 publications.
[IF=5.682] Ling Zuo. et al. Circular RNA circSHPRH inhibits the malignant behaviors of bladder cancer by regulating the miR-942/BARX2 pathway. Aging-Us. 2022 Feb 28; 14(4): 1891–1909 WB ; Human.
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| 研究领域 | 肿瘤 转录调节因子 细胞粘附分子 结合蛋白 细胞骨架 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human,Sheep,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 31 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human BarX2: 81-180/279 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the homeobox transcription factor family. A highly related protein in mouse has been shown to influence cellular processes that control cell adhesion and remodeling of the actin cytoskeleton in myoblast fusion and chondrogenesis. The encoded protein may also play a role in cancer progression. [provided by RefSeq, Jul 2008] Function: Transcription factor. Binds optimally to the DNA consensus sequence 5'-YYTAATGRTTTTY-3'. May control the expression of neural adhesion molecules such as L1 or Ng-CAM during embryonic development of both the central and peripherical nervous system. May be involved in controlling adhesive processes in keratinizing epithelia. Subcellular Location: Nucleus. Tissue Specificity: Highly expressed in adult salivary gland and at much lower levels in mammary gland, kidney and placenta. Post-translational modifications: Phosphorylated upon DNA damage, probably by ATM or ATR. Similarity: Belongs to the BAR homeobox family. Contains 1 homeobox DNA-binding domain. SWISS: Q9UMQ3 Gene ID: 8538 Database links: Entrez Gene: 8538 Human Entrez Gene: 12023 Mouse Omim: 604823 Human SwissProt: Q9UMQ3 Human SwissProt: O08686 Mouse Unigene: 591944 Human Unigene: 261044 Mouse |
| 产品图片 |
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti-BarX2 (bs-19273R) at 1/500 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 31 kD
Observed band size: 31 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse placenta); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (bs-19273R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Rat breast); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (BarX2) Polyclonal Antibody, Unconjugated (bs-19273R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
