| 产品编号 | bs-19247R |
| 英文名称 | NINL |
| 中文名称 | Ninein样蛋白抗体 |
| 别 名 | 4930519N13Rik; dJ691N24.1; Gm1004,; Gm1634; mKIAA0980; Ninein like; Ninein-like protein; NINL; NINL_HUMAN; Nlp; RGD1306152; RP23-193L22.3; RP4-691N24.1. |
| 研究领域 | 细胞生物 细胞周期蛋白 细胞分化 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | (predicted: Human,Mouse,Rat) |
| 产品应用 | IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 156kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NINL: 401-500/1382 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NINL is a 1,382 amino acid protein that localizes to both the cytoplasm and the centrosome and contains four EF-hand domains. Interacting with ?Tubulin, NLP is involved in microtubule organization in interphase cells and, when overexpressed, causes lysosomal dispersion and interferes with mitotic spindle assembly. NLP is subject to post-translational phosphorylation by Plk, an event which disrupts the association of NLP with centrosomes. Function: Involved in the microtubule organization in interphase cells. Overexpression induces the fragmentation of the Golgi, and causes lysosomes to disperse toward the cell periphery; it also interferes with mitotic spindle assembly. May play a role in ovarian carcinogenesis Subunit: nteracts with gamma-tubulin and TUBGCP4. Interacts with anaphase promoting complex/cyclosome (APC/C). Interacts with CDC20 and FZR1. Isoform 2 interacts with LCA5 and USH2A. Subcellular Location: Cytoplasm > cytoskeleton > centrosome. Cytoplasm. In interphase cells, NINL is transported to the centrosomes by the dynein-dynactin motor complex. During centrosome maturation, PLK1 directly phosphorylates NINL resulting in its release into the cytoplasm. Tissue Specificity: Expressed in KYSE150 esophageal carcinoma, HeLa cervical carcinoma and U2OS osteosarcoma cells. Expression is regulated in a cell cycle-dependent manner and peaks during G2/M phase (at protein level). Expressed in fetal heart, skeletal muscle, liver, lung and cochlea, and in adult brain, testis, kidney and retina. Post-translational modifications: Phosphorylated by PLK1 which disrupts its centrosome association and interaction with gamma-tubulin. Ubiquitinated by the APC/C complex leading to its degradation. Similarity: Contains 4 EF-hand domains. SWISS: Q9Y2I6 Gene ID: 22981 Database links: Entrez Gene: 100057245 Horse Entrez Gene: 22981 Human Entrez Gene: 78177 Mouse Omim: 609580 Human SwissProt: Q9Y2I6 Human SwissProt: Q6ZQ12 Mouse Unigene: 631508 Human Unigene: 120911 Mouse |
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
