| 产品编号 | bs-10704R |
| 英文名称 | NELL1 Rabbit pAb |
| 中文名称 | 蛋白激酶C结合蛋白NELL1抗体 |
| 别 名 | IDH3GL; NEL like protein 1; Nel related protein 1; NEL-like protein 1; Nel-related protein 1; Nell1; NELL1_HUMAN; Neural epidermal growth factor like 1; NRP1; Protein kinase C binding protein NELL1; Protein kinase C-binding protein NELL1. |
| 研究领域 | 细胞生物 神经生物学 信号转导 生长因子和激素 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 87 kDa |
| 检测分子量 | |
| 细胞定位 | 分泌型蛋白 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NELL1: 201-300/810 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cytoplasmic protein that contains epidermal growth factor (EGF)-like repeats. The encoded heterotrimeric protein may be involved in cell growth regulation and differentiation. A similar protein in rodents is involved in craniosynostosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013] Function: Involved in the control of cell growth and differentiation. Subunit: Homotrimer. Binds to PKC beta-1. Interacts with ATRAID; the interaction promotes osteoblast cell differentiation and mineralization. Subcellular Location: Secreted. Similarity: Contains 6 EGF-like domains. Contains 1 TSP N-terminal (TSPN) domain. Contains 5 VWFC domains. SWISS: Q92832 Gene ID: 4745 Database links: Entrez Gene: 4745 Human Entrez Gene: 338352 Mouse Omim: 602319 Human SwissProt: Q92832 Human SwissProt: Q2VWQ2 Mouse Unigene: 657172 Human Unigene: 383528 Mouse Unigene: 10695 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL1) Polyclonal Antibody, Unconjugated (bs-10704R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NELL1) Polyclonal Antibody, Unconjugated (bs-10704R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
