| 产品编号 | bs-19195R |
| 英文名称 | NEK11 Rabbit pAb |
| 中文名称 | 丝氨酸/苏氨酸蛋白激酶Nek11抗体 |
| 别 名 | 4932416N14Rik; FLJ23495; NEK11; NEK11_HUMAN; NEK11S/L; Never in mitosis A-related kinase 11; NIMA(never in mitosis gene a) related kinase 11; NIMA(never in mitosis gene a)-related kinase 11; NIMA related kinase 11L; NIMA related kinase 11S; NIMA-related kinase 11; NimA-related protein kinase 11; Serine/threonine-protein kinase Nek11. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 54 and 74 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NEK11: 151-250/645 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009] Function: Possible role in S-phase checkpoint mechanism. Subcellular Location: Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells. Tissue Specificity: Poorly expressed in cerebellum, trachea, lung, appendix, and uterus. Post-translational modifications: Phosphorylated. Similarity: Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily. Contains 1 protein kinase domain. SWISS: Q8NG66 Gene ID: 79858 Database links: Entrez Gene: 79858 Human Entrez Gene: 208583 Mouse Omim: 609779 Human SwissProt: Q8NG66 Human SwissProt: Q8C0Q4 Mouse Unigene: 657336 Human Unigene: 347509 Mouse |
| 产品图片 |
Sample: Hela (Human)Cell Lysate at 40 ug
Primary: Anti- NEK11(bs-19195R)at 1/300 dilution
Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution
Predicted band size: 54'74kD
Observed band size: 70kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK11) Polyclonal Antibody, Unconjugated (bs-19195R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
