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NEK11 Rabbit pAb (bs-19195R)  
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50ul/1180.00元
100ul/1980.00元
200ul/2800.00元
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产品编号 bs-19195R
英文名称 NEK11 Rabbit pAb
中文名称 丝氨酸/苏氨酸蛋白激酶Nek11抗体
别    名 4932416N14Rik; FLJ23495; NEK11; NEK11_HUMAN; NEK11S/L; Never in mitosis A-related kinase 11; NIMA(never in mitosis gene a) related kinase 11; NIMA(never in mitosis gene a)-related kinase 11; NIMA related kinase 11L; NIMA related kinase 11S; NIMA-related kinase 11; NimA-related protein kinase 11; Serine/threonine-protein kinase Nek11.  
研究领域 细胞生物  信号转导  激酶和磷酸酶  
抗体来源 Rabbit
克隆类型 Polyclonal
克 隆 号
交叉反应 Human,Mouse (predicted: Rat,Rabbit,Pig,Cow,Chicken,Dog,Horse)
产品应用 WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量 54 and 74 kDa
检测分子量
细胞定位 细胞核 
性    状 Liquid
浓    度 1mg/ml
免 疫 原 KLH conjugated synthetic peptide derived from human NEK11: 151-250/645 
亚    型 IgG
纯化方法 affinity purified by Protein A
缓 冲 液 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol.
保存条件 Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.
注意事项 This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMed PubMed
产品介绍 This gene encodes a member of the never in mitosis gene A family of kinases. The encoded protein localizes to the nucleoli, and may function with NEK2A in the S-phase checkpoint. The encoded protein appears to play roles in DNA replication and response to genotoxic stress. Alternatively spliced transcript variants have been described.[provided by RefSeq, Mar 2009]

Function:
Possible role in S-phase checkpoint mechanism.

Subcellular Location:
Nucleus. Nucleus > nucleolus. Nuclear during interphase but moves to the polar microtubules during prometaphase and metaphase. Accumulates in the nucleolus in G1/S-arrested cells.

Tissue Specificity:
Poorly expressed in cerebellum, trachea, lung, appendix, and uterus.

Post-translational modifications:
Phosphorylated.

Similarity:
Belongs to the protein kinase superfamily. NEK Ser/Thr protein kinase family. NIMA subfamily.
Contains 1 protein kinase domain.

SWISS:
Q8NG66

Gene ID:
79858

Database links:

Entrez Gene: 79858 Human

Entrez Gene: 208583 Mouse

Omim: 609779 Human

SwissProt: Q8NG66 Human

SwissProt: Q8C0Q4 Mouse

Unigene: 657336 Human

Unigene: 347509 Mouse



产品图片
Sample: Hela (Human)Cell Lysate at 40 ug Primary: Anti- NEK11(bs-19195R)at 1/300 dilution Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution Predicted band size: 54'74kD Observed band size: 70kD
Paraformaldehyde-fixed, paraffin embedded (Mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (NEK11) Polyclonal Antibody, Unconjugated (bs-19195R) at 1:500 overnight at 4°C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
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