| 产品编号 | bs-19182R |
| 英文名称 | Rabbit Anti-Prolyl Endopeptidase antibody |
| 中文名称 | 脯氨酰内肽酶抗体 |
| 别 名 | dJ355L5.1 (prolyl endopeptidase); HGNC:9358; MGC16060; OTTHUMP00000040498; PE; PEP; Post proline cleaving enzyme; Post-proline cleaving enzyme; PPCE_HUMAN; PREP; Prolyl endopeptidase; Prolyl oligopeptidase. |
| 研究领域 | 细胞生物 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Sheep,Cow,Chicken,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 81kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Prolyl Endopeptidase: 141-240/710 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
The protein encoded by this gene is a cytosolic prolyl endopeptidase that cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Prolyl endopeptidases have been reported to be involved in the maturation and degradation of peptide hormones and neuropeptides. [provided by RefSeq, Jul 2008] Function: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Tissue Specificity: Cytoplasm. Post-translational modifications: The N-terminus is blocked. Similarity: Belongs to the peptidase S9A family. SWISS: P48147 Gene ID: 5550 Database links: Entrez Gene: 5550 Human Entrez Gene: 421785 Chicken Entrez Gene: 19072 Mouse Omim: 600400 Human SwissProt: P48147 Human SwissProt: Q9QUR6 Mouse Unigene: 436564 Human Unigene: 37294 Mouse Unigene: 11058 Rat |
| 产品图片 |
Sample:
NIH/3T3(Mouse) Cell Lysate at 30 ug
Primary: Anti-Prolyl Endopeptidase (bs-19182R) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 81 kD
Observed band size: 81 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
