| 产品编号 | bs-19164R |
| 英文名称 | MYO16 Rabbit pAb |
| 中文名称 | 肌球蛋白16/Myosin XVI抗体 |
| 别 名 | MYAP3; MYR8; Myo16b; NYAP3; PPP1R107; C230040D10Rik; Gm1105; mKIAA0865; MYO16_HUMAN; MYO16; Neuronal tyrosine-phosphorylated phosphoinositide-3-kinase adapter 3; Unconventional myosin-16; KIAA0865; MYO16_MOUSE; MYO16_RAT; Myosin heavy chain myr 8; |
| 研究领域 | 细胞生物 信号转导 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse,Rat (predicted: Human,Zebrafish,Chicken,Fruit Fly) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 206 kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MYO16: 1251-1350/1858 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Myosin XVI is a 1,858 amino acid protein that localizes to the cytoplasm and contains one IQ domain, two myosin head-like domains and seven ANK repeats. Existing as an unconventional Myosin, Myosin XVI is involved in intracellular movements related to Actin filaments and is thought to interact specifically with PP1 alpha and PP1 gamma, possibly playing a role in PP1-associated brain development. Multiple isoforms of Myosin XVI exist due to alternative splicing events. Subcellular Location: Cytoplasm. Found in puncta in soma and processes of astrocytes and dissociated cerebellar cells with the morphology of migrating granule cells. Similarity: Contains 7 ANK repeats. Contains 1 IQ domain. Contains 2 myosin head-like domains. SWISS: Q9Y6X6 Gene ID: 23026 Database links: Entrez Gene: 23026 Human Entrez Gene: 244281 Mouse SwissProt: Q9Y6X6 Human SwissProt: Q5DU14 Mouse Unigene: 656587 Human Unigene: 422761 Mouse Unigene: 207204 Rat |
| 产品图片 |
Sample:
Brain (Mouse) Lysate at 40 ug
Brain (Rat) Lysate at 40 ug
Primary: Anti-MYO16 (bs-19164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 206 kD
Observed band size: 123 kD
Sample:
Cerebrum (Mouse) Lysate at 40 ug
Cerebrum (Rat) Lysate at 40 ug
Primary: Anti-MYO16 (bs-19164R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 206 kD
Observed band size: 123 kD
Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MYO16) Polyclonal Antibody, Unconjugated (bs-19164R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
