| 产品编号 | bs-19059R |
| 英文名称 | NDFIP2 Rabbit pAb |
| 中文名称 | NDFIP2抗体 |
| 别 名 | Ndfip2; NEDD4 family interacting protein 2; NEDD4 family-interacting protein 2; NEDD4 WW domain binding protein 5A; NEDD4 WW domain-binding protein 5A; NFIP2_HUMAN; Putative MAPK activating protein PM04/PM05/PM06/PM07; Putative MAPK-activating protein PM0 |
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Specific References (1) | bs-19059R has been referenced in 1 publications.
[IF=5.722] Zhou, Yongqiang. et al. Gly-tRF enhances LCSC-like properties and promotes HCC cells migration by targeting NDFIP2. Cancer Cell Int. 2021 Dec;21(1):1-16 WB,IF ; human.
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| 研究领域 | 细胞生物 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human (predicted: Mouse,Rat,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 36 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human NDFIP2: 261-336/336 <Extracellular> |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
NDFIP2 is a 336 amino acid protein that localizes to the membrane of both the endosome and the golgi apparatus. Expressed in kidney, heart, brain, lung, liver, placenta and skeletal muscle, Ndfip2 interacts with NEDD4 and NEDD4-L and is thought to be involved in endocytosis and in the NFⅹB and MAPK signaling pathways. Additionally, Ndfip2 may function as an adaptor protein that may recruit NEDD4 ubiquitin-protein ligases to protein trafficking machinery in the golgi. Ndfip2 is activated by T cells and may be ubiquitinated by NEDD4 or NEDD4-L, an event that does not affect Ndfip2 function. The gene encoding Ndfip2 maps to human chromosome 13, which houses over 400 genes, such as BRCA2 and RB1, and comprises nearly 4% of the human genome. Function: Activates HECT domain-containing E3 ubiquitin-protein ligases, including ITCH, NEDD4, NEDD4L, SMURF2, WWP1 and WWP2, and consequently modulates the stability of their targets. As a result, may control many cellular processes. Recruits ITCH, NEDD4 and SMURF2 to endosomal membranes. May modulate EGFR signaling. Subunit: Forms heterodimers with NDFIP1. Interacts with HECT domain-containing E3 ubiquitin-protein ligases, including NEDD4. Interacts with NEDD4L By similarity. Interacts with PTEN. When phosphorylated at Tyr-167, interacts with SRC and LYN SH2 domain. May thus act as a scaffold that recruits SRC to NDFIP1, enhancing NDFIP1 phosphorylation. Interacts with SLC11A2/DMT1. May interact with phosphorylated EGFR. Subcellular Location: Endosome membrane. Golgi apparatus membrane. Endosome > multivesicular body membrane. Tissue Specificity: Expressed in brain, lung, heart, skeletal muscle, kidney, liver and placenta. Post-translational modifications: Ubiquitinated by NEDD4 and ITCH. Also ubiquitinated by NEDD4L. Ubiquitination by NEDD4 or NEDD4L does not affect turnover. Undergoes transient tyrosine-phosphorylation following EGF stimulation, most probably catalyzed by SRC. Also phosphorylated by LYN and FYN. Gene ID: 54602 Database links: Entrez Gene: 76273 Mouse SwissProt: Q91ZP6 Mouse Unigene: 290669 Mouse Unigene: 387487 Mouse |
| 产品图片 |
Sample: Siha Cell (Human) Lysate at 40 ug
Primary: Anti- NDFIP2 (bs-19059R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 36 kD
Observed band size: 34 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
