| 产品编号 | bs-19005R |
| 英文名称 | Naked1 Rabbit pAb |
| 中文名称 | Naked1蛋白抗体 |
| 别 名 | Dvl-binding protein; Naked cuticle 1; naked cuticle 1 homolog; Naked cuticle homolog 1(Drosophila); Naked-1; NKD; Nkd1; Protein naked cuticle homolog 1. |
| 研究领域 | 细胞生物 免疫学 信号转导 干细胞 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Dog,Horse) |
| 产品应用 | WB=1:500-2000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 52 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Naked1: 301-400/470 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
In the mouse, Nkd is a Dishevelled (see DVL1; MIM 601365)-binding protein that functions as a negative regulator of the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf (see MIM 602272) signaling pathway.[supplied by OMIM, Jun 2003] Function: Cell autonomous antagonist of the canonical Wnt signaling pathway. May activate a second Wnt signaling pathway that controls planar cell polarity. Subunit: Interacts with DVL1, DVL2, DVL3 and PPP2R3A. Subcellular Location: Cell membrane By similarity. Cytoplasm Tissue Specificity: Expressed in colon, heart, kidney, leukocyte, liver, lung, ovary, pancreas, placenta, prostate, skeletal muscle, small intestine and spleen. Similarity: Belongs to the NKD family. Contains 1 EF-hand domain. SWISS: Q969G9 Gene ID: 85407 Database links: Entrez Gene: 85407 Human Entrez Gene: 93960 Mouse Omim: 607851 Human SwissProt: Q969G9 Human SwissProt: Q99MH6 Mouse Unigene: 187578 Human Unigene: 30219 Mouse |
| 产品图片 |
Sample: Heart (Mouse) Lysate at 40 ug
Primary: Anti-Naked1 (bs-19005R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 52 kD
Observed band size: 52 kD
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
