| 产品编号 | bs-18922R |
| 英文名称 | TMEM175 |
| 中文名称 | 跨膜蛋白175抗体 |
| 别 名 | MGC4618; Hypothetical protein DKFZp547K246; hypothetical protein LOC84286; Hypothetical protein MGC4618; TM175_HUMAN; tmem175; Transmembrane protein 175. |
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Specific References (1) | bs-18922R has been referenced in 1 publications.
[IF=5.682] Palomba, Nicole Piera. et al. Common and Rare Variants in TMEM175 Gene Concur to the Pathogenesis of Parkinson’s Disease in Italian Patients. MOL NEUROBIOL. 2023 Jan;:1-24 IHC ; Mouse.
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| 研究领域 | 细胞生物 免疫学 跨膜蛋白 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse,Rat (predicted: Human) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500, ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 56kDa |
| 细胞定位 | 细胞膜 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human TM175: 231-330/504 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
TMEM175 (Transmembrane Protein 175) is a Protein Coding gene. Function: Organelle-specific potassium channel specifically responsible for potassium conductance in endosomes and lysosomes. Forms a potassium-permeable leak-like channel, which regulates lumenal pH stability and is required for autophagosome-lysosome fusion. Constitutes the major lysosomal potassium channel. Subunit: Homodimer. Subcellular Location: Membrane. Tissue Specificity: Widely expressed. Similarity: Belongs to the TMEM175 family. SWISS: Q9BSA9 Gene ID: 84286 Database links: Entrez Gene: 84286 Human Entrez Gene: 72392 Mouse SwissProt: Q9BSA9 Human SwissProt: Q9CXY1 Mouse Unigene: 478936 Human Unigene: 258324 Mouse Unigene: 159765 Rat |
| 产品图片 |
Sample:
Lane 1: Cerebrum (Mouse) Lysate at 40 ug Lane 2: Kidney (Mouse) Lysate at 40 ug Lane 3: Liver (Mouse) Lysate at 40 ug Lane 4: Testis (Mouse) Lysate at 40 ug Lane 5: Lung (Mouse) Lysate at 40 ug Lane 6: Spleen (Mouse) Lysate at 40 ug Lane 7: Cerebral cortex (Rat) Lysate at 40 ug Lane 8: Liver (Rat) Lysate at 40 ug Lane 9: Testis (Rat) Lysate at 40 ug Primary: Anti-TMEM175 (bs-18922R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 56/43 kD Observed band size: 45 kD 
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM175) Polyclonal Antibody, Unconjugated (bs-18922R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Paraformaldehyde-fixed, paraffin embedded (mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (TMEM175) Polyclonal Antibody, Unconjugated (bs-18922R) at 1:2000 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
