| 产品编号 | bs-18796R |
| 英文名称 | MESP2 Rabbit pAb |
| 中文名称 | 碱性螺旋环螺旋蛋白MESP2抗体 |
| 别 名 | Basic helix loop helix protein MESP 2; Basic helix loop helix protein MESP2; BHLH protein MesP2; bHLHc6; Class C basic helix-loop-helix protein 6; Hypothetical class II basic helix loop helix protein MESP 2; Hypothetical class II basic helix loop helix protein MESP2; Mesoderm posterior 2; mesoderm posterior 2 homolog(mouse); Mesoderm posterior 2 homolog; Mesoderm posterior protein 2; Mesp 2; MESP2; MESP2_HUMAN; SCDO 2; SCDO2. |
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Specific References (1) | bs-18796R has been referenced in 1 publications.
[IF=7.109] Ge, Lingjun. et al. MESP2 binds competitively to TCF4 to suppress gastric cancer progression by regulating the SKP2/p27 axis. CELL DEATH DISCOV. 2023 Mar;9(1):1-13 IHC ; Human.
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| 研究领域 | 细胞生物 神经生物学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Rat (predicted: Human,Mouse,Dog) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 42 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MESP2: 311-397/397 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a member of the bHLH family of transcription factors and plays a key role in defining the rostrocaudal patterning of somites via interactions with multiple Notch signaling pathways. This gene is expressed in the anterior presomitic mesoderm and is downregulated immediately after the formation of segmented somites. This gene also plays a role in the formation of epithelial somitic mesoderm and cardiac mesoderm. Mutations in the MESP2 gene cause autosomal recessive spondylocostal dystosis 2 (SCD02). [provided by RefSeq, Oct 2008] Function: Transcription factor with important role in somitogenesis. Defines the rostrocaudal patterning of the somite by participating in distinct Notch pathways. Regulates also the FGF signaling pathway. Specifies the rostral half of the somites. Generates rostro-caudal polarity of somites by down-regulating in the presumptive rostral domain DLL1, a Notch ligand. Participates in the segment border formation by activating in the anterior presomitic mesoderm LFNG, a negative regulator of DLL1-Notch signaling. Acts as a strong suppressor of Notch activity. Together with MESP1 is involved in the epithelialization of somitic mesoderm and in the development of cardiac mesoderm. Subcellular Location: Nucleus. Post-translational modifications: Degraded by the proteasome. DISEASE: Defects in MESP2 are the cause of spondylocostal dysostosis type 2 (SCDO2) [MIM:608681]. An autosomal recessive condition of variable severity associated with vertebral and rib segmentation defects. The main skeletal malformations include fusion of vertebrae, hemivertebrae, fusion of certain ribs, and other rib malformations. Deformity of the chest and spine (severe scoliosis, kyphoscoliosis and lordosis) is a natural consequence of the malformation and leads to a dwarf-like appearance. As the thorax is small, infants frequently have respiratory insufficiency and repeated respiratory infections resulting in life-threatening complications in the first year of life. Similarity: Contains 1 bHLH (basic helix-loop-helix) domain. SWISS: Q0VG99 Gene ID: 145873 Database links: Entrez Gene: 145873 Human Omim: 605195 Human SwissProt: Q0VG99 Human Unigene: 37311 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Rat liver); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MESP2) Polyclonal Antibody, Unconjugated (bs-18796R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
