| 产品编号 | bs-18771R |
| 英文名称 | MED4 Rabbit pAb |
| 中文名称 | MED4蛋白抗体 |
| 别 名 | Activator-recruited cofactor 36 kDa component; ARC36; DRIP36; FLJ10956; HSPC126; med4; MED4_HUMAN; Mediator complex subunit 4; Mediator of RNA polymerase II transcription subunit 4; Mediator of RNA polymerase II transcription, subunit 4 homolog; OTTHUMP00000018390; OTTHUMP00000018391; OTTHUMP00000018392; RP11-90M2.2; TRAP/SMCC/PC2 subunit p36 subunit; TRAP36; VDRIP; Vitamin D3 receptor-interacting protein complex 36 kDa component. |
| 研究领域 | 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Rat (predicted: Mouse,Rabbit,Pig,Sheep,Cow,Chicken,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 30 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MED4: 101-200/270 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a component of the Mediator complex. The Mediator complex interacts with DNA-binding gene-specific transcription factors to modulate transcription by RNA polymerase II. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Jul 2012] Function: Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Subcellular Location: Nucleus. Similarity: Belongs to the Mediator complex subunit 4 family. SWISS: Q9NPJ6 Gene ID: 29079 Database links: Entrez Gene: 29079 Human Entrez Gene: 418859 Chicken Entrez Gene: 67381 Mouse Entrez Gene: 550582 Zebrafish Omim: 605718 Human SwissProt: Q9NPJ6 Human SwissProt: Q3UL96 Mouse SwissProt: Q8BVG6 Mouse SwissProt: Q9CQA5 Mouse SwissProt: Q561X3 Zebrafish Unigene: 181112 Human Unigene: 282888 Mouse Unigene: 114469 Rat Unigene: 84720 Zebrafish |
| 产品图片 |
Sample: A549 Cell (Human) Lysate at 30 ug
Primary: Anti- MED4 (bs-18771R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 30kD
Observed band size: 30kD
Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MED4) Polyclonal Antibody, Unconjugated (bs-18771R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
