| 产品编号 | bs-18555R |
| 英文名称 | ZCCHC6 |
| 中文名称 | ZCCHC6蛋白抗体 |
| 别 名 | KIAA1711; MGC38585; PAP associated domain containing 6; PAPD6; Zinc finger CCHC domain containing 6. |
| 研究领域 | 转录调节因子 锌指蛋白 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Mouse (predicted: Human,Rat,Dog,Pig,Rabbit) |
| 产品应用 | WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, ICC=1:100-500, IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 171kDa |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human ZCCHC6: 111-210/1495 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
ZCCHC6 is a 1,495 amino acid uridylyltransferase that mediates RNA uridylation. A member of the DNA polymerase type-B-like family, ZCCHC6 contains three CCHC-type zinc fingers and two PAP-associated domains, and exists as six alternatively spliced isoforms. The gene encoding ZCCHC6 maps to human chromosome 9q21.33 and mouse chromosome 13 B2. Function: Uridylyltransferase that mediates the terminal uridylation of some specific RNAs. Not involved in uridylation of precursor let-7 (pre-let-7) miRNA. Does not play a role in replication-dependent histone mRNA degradation. Similarity: Belongs to the DNA polymerase type-B-like family. Contains 3 CCHC-type zinc fingers. Contains 2 PAP-associated domains. SWISS: Q5VYS8 Gene ID: 79670 Database links: Entrez Gene: 79670 Human SwissProt: Q5VYS8 Human |
| 产品图片 |
Sample:
Liver (Mouse) Lysate at 40 ug Primary: Anti-ZCCHC6 (bs-18555R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 171 kD Observed band size: 171 kD 
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (ZCCHC6) Polyclonal Antibody, Unconjugated (bs-18555R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
