| 产品编号 | bs-18448R |
| 英文名称 | LW1/HSFX1 Rabbit pAb |
| 中文名称 | 热休克转录因子家族X连锁1抗体 |
| 别 名 | hCG_27154; heat shock transcription factor family X linked 1; Heat shock transcription factor, X-linked; HSFX1; HSFX2; LW 1; LW1. |
| 研究领域 | 细胞生物 免疫学 信号转导 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 45 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞核 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human LW1/HSFX1: 181-280/423 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
HSFX1 (heat shock transcription factor family, X linked 1), also known as LW-1, is a 423 amino acid protein that is predominately expressed in testis. Localized to the cytoplasm, HSFX1 is thought to be involved in spermatogenesis and male fertility. Subcellular Location: Nucleus (Potential). Cytoplasm. Tissue Specificity: Testis-specific. Similarity: Belongs to the HSF family. SWISS: Q9UBD0 Gene ID: 100506164 Database links: Entrez Gene: 100506164 Human GenBank: NM_016153 Human SwissProt: Q9UBD0 Human Unigene: 709146 Human |
| 产品图片 |
Sample:
Jurkat Cell (Human) Lysate at 40 ug
Hela Cell (Human) Lysate at 40 ug
Primary: Anti-LW1/HSFX1 (bs-18448R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 45 kD
Observed band size: 48 kD
Paraformaldehyde-fixed, paraffin embedded (Human liver carcinoma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (HSFX1) Polyclonal Antibody, Unconjugated (bs-18448R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
|
| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
