| 产品编号 | bs-18331R |
| 英文名称 | Rabbit Anti-SAKS1/2B28 antibody |
| 中文名称 | 丝裂原活化蛋白激酶底物1抗体 |
| 别 名 | LOC51035 2B28; 4930455J02Rik; D19Ertd721e; MGC124706; MGC6696; mY33K; UBXN1_HUMAN; Protein 2B28; RGD1309471; SAKS1; SAPK substrate protein 1; T25529; UBA/UBX 33.3 kDa protein; UBX domain containing protein 1; UBX domain protein 1; UBXD10; UBXN1. |
| 研究领域 | 细胞生物 信号转导 激酶和磷酸酶 泛素 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Rat (predicted: Human,Mouse,Pig,Sheep,Cow,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,ICC/IF=1:100-500,IF=1:100-500,ELISA=1:5000-10000
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 33kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human SAKS1/2B28: 51-150/297 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
2B28 is a component of a complex composed of NGLY1, SAKS1, AMFR, VCP and RAD23B, where it acts as an adapter that directs VCP to polyubiquitinated proteins. 2B28 also interacts with Homer 2, which further suggests role for 2B28 in the regulation of protein degradation by ubiquitin-proteasome systems. Function: Component of a complex required to couple deglycosylation and proteasome-mediated degradation of misfolded proteins in the endoplasmic reticulum that are retrotranslocated in the cytosol. Involved in ubiquitin-proteasome systems. 2 named isoforms exist. Subunit: Component of a complex required to couple retrotranslocation, ubiquitination and deglycosylation composed of NGLY1, SAKS1, AMFR, VCP and RAD23B. Interacts with HOMER2 (By similarity). Interacts directly with VCP. Interacts with BRCA1 and BARD1; interaction takes place when BRCA1 is not autoubiquitinated but is strongly enhanced in the presence of autoubiquitinated BRCA1. Subcellular Location: Cytoplasm Similarity: Belongs to the mitochondrial carrier (TC 2.A.29) family. SWISS: Q04323 Gene ID: 51035 Database links: Entrez Gene: 51035 Human Entrez Gene: 225896 Mouse SwissProt: Q04323 Human SwissProt: Q922Y1 Mouse Unigene: 351296 Human Unigene: 27839 Mouse Unigene: 198539 Rat |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (rat uterus); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (SAKS1 2B28) Polyclonal Antibody, Unconjugated (bs-18331R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
