| 产品编号 | bs-18227R |
| 英文名称 | Leucyl tRNA synthetase Rabbit pAb |
| 中文名称 | 胞浆亮氨酸tRNA连接酶抗体 |
| 别 名 | Cytoplasmic leucyl tRNA synthetase; Cytosolic leucyl tRNA synthetase; FLJ10595; FLJ21788; hr025Cl; HSPC 192; HSPC192; KIAA1352; LARS 1; LARS; LARS1; Leucine translase; Leucine tRNA ligase; Leucine tRNA ligase; Leucyl tRNA synthetase cytoplasmic; LeuRS; LEUS; LRS; PIG 44; PIG44; Proliferation inducing gene 44; RNTLS. |
| 研究领域 | 细胞生物 免疫学 转录调节因子 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Human,Mouse (predicted: Rat,Rabbit,Pig,Dog,Horse) |
| 产品应用 | IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 135 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human Leucyl tRNA synthetase: 201-300/1176 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
This gene encodes a cytosolic leucine-tRNA synthetase, a member of the class I aminoacyl-tRNA synthetase family. The encoded enzyme catalyzes the ATP-dependent ligation of L-leucine to tRNA(Leu). It is found in the cytoplasm as part of a multisynthetase complex and interacts with the arginine tRNA synthetase through its C-terminal domain. Alternatively spliced transcript variants of this gene have been found; however, their full-length nature is not known. [provided by RefSeq, Jul 2008] Function: Leucyl tRNA synthetase is a component of multi ARS complex and catalyzes the ligation of leucine with leucyl tRNA. Subcellular Location: Cytoplasmic Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. SWISS: Q9P2J5 Gene ID: 51520 Database links: Entrez Gene: 51520 Human Entrez Gene: 107045 Mouse Omim: 151350 Human SwissProt: Q9P2J5 Human SwissProt: Q8BMJ2 Mouse Unigene: 432674 Human |
| 产品图片 |
Paraformaldehyde-fixed, paraffin embedded (Human colorectal cancer); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-18227R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Leucyl tRNA synthetase) Polyclonal Antibody, Unconjugated (bs-18227R) at 1:400 overnight at 4°C, followed by a conjugated secondary antibody (sp-0023) for 20 minutes and DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
