| 产品编号 | bs-17893R |
| 英文名称 | MTRF1L Rabbit pAb |
| 中文名称 | 线粒体释放因子1样蛋白抗体 |
| 别 名 | HMRF1L; Human mitochondrial release factor 1-like; Mitochondrial release factor 1 like; Mitochondrial translational release factor 1 like; Mitochondrial translational release factor 1-like; mtRF1a; MTRF1L; Peptide chain release factor 1-like, mitochondrial; RF1ML_HUMAN. |
| 研究领域 | 细胞生物 转录调节因子 线粒体 表观遗传学 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 克 隆 号 | |
| 交叉反应 | Mouse (predicted: Human,Rat,Rabbit,Pig,Cow,Dog,Horse) |
| 产品应用 | WB=1:500-2000,IHC-P=1:100-500,IHC-F=1:100-500,IF=1:100-500
not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 理论分子量 | 40 kDa |
| 检测分子量 | |
| 细胞定位 | 细胞浆 线粒体 |
| 性 状 | Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human MTRF1L: 301-380/380 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 缓 冲 液 | 0.01M TBS (pH7.4) with 1% BSA, 0.02% Proclin300 and 50% Glycerol. |
| 保存条件 | Shipped at 4℃. Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. |
| 注意事项 | This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. |
| PubMed | PubMed |
| 产品介绍 |
Mitochondrial DNA encodes 13 proteins that play essential roles in the respiratory chain, while all proteins involved in mitochondrial translation are encoded by nuclear genes that are imported from the cytoplasm. MTRF1L is a nuclear-encoded protein that functions as a releasing factor that recognizes termination codons and releases mitochondrial ribosomes from the synthesized protein (summary by Nozaki et al., 2008 [PubMed 18429816]).[supplied by OMIM, Nov 2010] Function: Mitochondrial peptide chain release factor that directs the termination of translation in response to the peptide chain termination codons UAA and UAG. Subcellular Location: Mitochondrion. Tissue Specificity: Expressed in skeletal muscle (at protein level). Post-translational modifications: Methylation of glutamine in the GGQ triplet is conserved from bacteria to mammals. Similarity: Belongs to the prokaryotic/mitochondrial release factor family. SWISS: Q9UGC7 Gene ID: 54516 Database links: Entrez Gene: 54516 Human Omim: 613542 Human SwissProt: Q9UGC7 Human Unigene: 225836 Human |
| 产品图片 |
Sample:
Muscle (Mouse) Lysate at 40 ug
Primary: Anti-MTRF1 (bs-17893R) at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 40 kD
Observed band size: 43 kD
Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MTRF1) Polyclonal Antibody, Unconjugated (bs-17893R) at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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| 1、抗体溶解方法 | |
| 2、抗体修复方式 | |
| 3、常用试剂的配制 | |
| 4、免疫组化操作步骤 | |
| 5、免疫组化问题解答 | |
| 6、Western Blotting 操作步骤 | |
| 7、Western Blotting 问题解答 | |
| 8、关于肽链的设计 | |
| 9、多肽的溶解与保存 | |
| 10、酶标抗体效价测定程序 | |
